The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: Highlights from a multi-level omics approach

2.50
Hdl Handle:
http://hdl.handle.net/10033/620794
Title:
The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: Highlights from a multi-level omics approach
Authors:
Poblete-Castro, Ignacio; Escapa, Isabel F; Jäger, Christian; Puchalka, Jacek; Chi Lam, Carolyn M; Schomburg, Dietmar; Prieto, María A; Martins dos Santos, Vítor A
Abstract:
Abstract Background Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures. Results We found that 26, 62, and 81% of the cell dry weight consist of PHA under conditions of carbon, dual, and nitrogen limitation, respectively. Under nitrogen limitation a specific PHA production rate of 0.43 (g·(g·h)-1) was obtained. The residual biomass was not constant for dual- and strict nitrogen-limiting growth, showing a different feature in comparison to other P. putida strains. Dual limitation resulted in patterns of gene expression, protein level, and metabolite concentrations that substantially differ from those observed under exclusive carbon or nitrogen limitation. The most pronounced differences were found in the energy metabolism, fatty acid metabolism, as well as stress proteins and enzymes belonging to the transport system. Conclusion This is the first study where the interrelationship between nutrient limitations and PHA synthesis has been investigated under well-controlled conditions using a system level approach. The knowledge generated will be of great assistance for the development of bioprocesses and further metabolic engineering work in this versatile organism to both enhance and diversify the industrial production of PHAs.
Citation:
Microbial Cell Factories. 2012 Mar 20;11(1):34
Issue Date:
20-Mar-2012
URI:
http://dx.doi.org/10.1186/1475-2859-11-34; http://hdl.handle.net/10033/620794
Type:
Journal Article
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorPoblete-Castro, Ignacioen
dc.contributor.authorEscapa, Isabel Fen
dc.contributor.authorJäger, Christianen
dc.contributor.authorPuchalka, Jaceken
dc.contributor.authorChi Lam, Carolyn Men
dc.contributor.authorSchomburg, Dietmaren
dc.contributor.authorPrieto, María Aen
dc.contributor.authorMartins dos Santos, Vítor Aen
dc.date.accessioned2017-01-30T15:27:39Z-
dc.date.available2017-01-30T15:27:39Z-
dc.date.issued2012-03-20en
dc.identifier.citationMicrobial Cell Factories. 2012 Mar 20;11(1):34en
dc.identifier.urihttp://dx.doi.org/10.1186/1475-2859-11-34en
dc.identifier.urihttp://hdl.handle.net/10033/620794-
dc.description.abstractAbstract Background Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures. Results We found that 26, 62, and 81% of the cell dry weight consist of PHA under conditions of carbon, dual, and nitrogen limitation, respectively. Under nitrogen limitation a specific PHA production rate of 0.43 (g·(g·h)-1) was obtained. The residual biomass was not constant for dual- and strict nitrogen-limiting growth, showing a different feature in comparison to other P. putida strains. Dual limitation resulted in patterns of gene expression, protein level, and metabolite concentrations that substantially differ from those observed under exclusive carbon or nitrogen limitation. The most pronounced differences were found in the energy metabolism, fatty acid metabolism, as well as stress proteins and enzymes belonging to the transport system. Conclusion This is the first study where the interrelationship between nutrient limitations and PHA synthesis has been investigated under well-controlled conditions using a system level approach. The knowledge generated will be of great assistance for the development of bioprocesses and further metabolic engineering work in this versatile organism to both enhance and diversify the industrial production of PHAs.en
dc.titleThe metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: Highlights from a multi-level omics approachen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderPoblete-Castro et al; licensee BioMed Central Ltd.en
dc.date.updated2015-09-04T08:26:49Zen
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