Dynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulation

2.50
Hdl Handle:
http://hdl.handle.net/10033/620795
Title:
Dynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulation
Authors:
He, Feng; Buer, Jan; Zeng, An-Ping; Balling, Rudi
Abstract:
Abstract Background The regulation of genes in multicellular organisms is generally achieved through the combinatorial activity of different transcription factors. However, the quantitative mechanisms of how a combination of transcription factors controls the expression of their target genes remain unknown. Results By using the information on the yeast transcription network and high-resolution time-series data, the combinatorial expression profiles of regulators that best correlate with the expression of their target genes are identified. We demonstrate that a number of factors, particularly time-shifts among the different regulators as well as conversion efficiencies of transcription factor mRNAs into functional binding regulators, play a key role in the quantification of target gene expression. By quantifying and integrating these factors, we have found a highly significant correlation between the combinatorial time-series expression profile of regulators and their target gene expression in 67.1% of the 161 known yeast three-regulator motifs and in 32.9% of 544 two-regulator motifs. For network motifs involved in the cell cycle, these percentages are much higher. Furthermore, the results have been verified with a high consistency in a second independent set of time-series data. Additional support comes from the finding that a high percentage of motifs again show a significant correlation in time-series data from stress-response studies. Conclusion Our data strongly support the concept that dynamic cumulative regulation is a major principle of quantitative transcriptional control. The proposed concept might also apply to other organisms and could be relevant for a wide range of biotechnological applications in which quantitative gene regulation plays a role.
Citation:
Genome Biology. 2007 Sep 04;8(9):R181
Issue Date:
4-Sep-2007
URI:
http://dx.doi.org/10.1186/gb-2007-8-9-r181; http://hdl.handle.net/10033/620795
Type:
Journal Article
Appears in Collections:
Publications of Scientific Director (GFW)

Full metadata record

DC FieldValue Language
dc.contributor.authorHe, Fengen
dc.contributor.authorBuer, Janen
dc.contributor.authorZeng, An-Pingen
dc.contributor.authorBalling, Rudien
dc.date.accessioned2017-01-30T15:31:26Z-
dc.date.available2017-01-30T15:31:26Z-
dc.date.issued2007-09-04en
dc.identifier.citationGenome Biology. 2007 Sep 04;8(9):R181en
dc.identifier.urihttp://dx.doi.org/10.1186/gb-2007-8-9-r181en
dc.identifier.urihttp://hdl.handle.net/10033/620795-
dc.description.abstractAbstract Background The regulation of genes in multicellular organisms is generally achieved through the combinatorial activity of different transcription factors. However, the quantitative mechanisms of how a combination of transcription factors controls the expression of their target genes remain unknown. Results By using the information on the yeast transcription network and high-resolution time-series data, the combinatorial expression profiles of regulators that best correlate with the expression of their target genes are identified. We demonstrate that a number of factors, particularly time-shifts among the different regulators as well as conversion efficiencies of transcription factor mRNAs into functional binding regulators, play a key role in the quantification of target gene expression. By quantifying and integrating these factors, we have found a highly significant correlation between the combinatorial time-series expression profile of regulators and their target gene expression in 67.1% of the 161 known yeast three-regulator motifs and in 32.9% of 544 two-regulator motifs. For network motifs involved in the cell cycle, these percentages are much higher. Furthermore, the results have been verified with a high consistency in a second independent set of time-series data. Additional support comes from the finding that a high percentage of motifs again show a significant correlation in time-series data from stress-response studies. Conclusion Our data strongly support the concept that dynamic cumulative regulation is a major principle of quantitative transcriptional control. The proposed concept might also apply to other organisms and could be relevant for a wide range of biotechnological applications in which quantitative gene regulation plays a role.en
dc.titleDynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulationen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderHe et al..en
dc.date.updated2015-09-04T08:25:45Zen
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