Temperature-dependent growth modeling of environmental and clinical Legionella pneumophila MLVA-genotypes.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620840
Title:
Temperature-dependent growth modeling of environmental and clinical Legionella pneumophila MLVA-genotypes.
Authors:
Sharaby, Yehonatan; Rodríguez-Martínez, Sarah; Oks, Olga; Pecellin, Marina; Mizrahi, Hila; Peretz, Avi; Brettar, Ingrid; Höfle, Manfred; Halpern, Malka
Abstract:
Legionella pneumophila cause waterborne infections resulting in severe pneumonia. High resolution genotyping of L. pneumophila isolates can be achieved by Multiple-Locus Variable number of tandem repeat Analysis (MLVA). Recently, we found that different MLVA genotypes of L. pneumophila, dominated different sites in a small drinking-water network, with a genotype-related temperature and abundance regime. The current study focuses on understanding the temperature-dependent growth kinetics of the genotypes that dominated the water network. Our aim was to model mathematically the influence of temperature on the growth kinetics of different environmental and clinical L. pneumophila genotypes and compare it with their ecological niches. Environmental strains showed a distinct temperature preference with significant differences among the growth kinetics of the three studied genotypes (Gt4, Gt6 and Gt15). Gt4 strains exhibited superior growth at lower temperatures (25-30 °C) while Gt15 strains appeared to be best adapted to relatively higher temperatures (42-45 °C). The temperature-dependent growth traits of the environmental genotypes were consistent with their distribution and temperature preferences in the water network. Clinical isolates exhibited significantly higher growth rates and reached higher maximal cell densities at 37°C and 42°C than the environmental strains. Further research on the growth preferences of L. pneumophila clinical and environmental genotypes will result in better understanding of their ecological niches in drinking water systems as well as in the human body.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Temperature-dependent growth modeling of environmental and clinical Legionella pneumophila MLVA-genotypes. 2017 Appl. Environ. Microbiol.
Journal:
Applied and environmental microbiology
Issue Date:
3-Feb-2017
URI:
http://hdl.handle.net/10033/620840
DOI:
10.1128/AEM.03295-16
PubMed ID:
28159784
Type:
Article
Language:
en
ISSN:
1098-5336
Appears in Collections:
publications of the research group microbial diagnosis (MIDI)

Full metadata record

DC FieldValue Language
dc.contributor.authorSharaby, Yehonatanen
dc.contributor.authorRodríguez-Martínez, Sarahen
dc.contributor.authorOks, Olgaen
dc.contributor.authorPecellin, Marinaen
dc.contributor.authorMizrahi, Hilaen
dc.contributor.authorPeretz, Avien
dc.contributor.authorBrettar, Ingriden
dc.contributor.authorHöfle, Manfreden
dc.contributor.authorHalpern, Malkaen
dc.date.accessioned2017-02-23T13:58:26Z-
dc.date.available2017-02-23T13:58:26Z-
dc.date.issued2017-02-03-
dc.identifier.citationTemperature-dependent growth modeling of environmental and clinical Legionella pneumophila MLVA-genotypes. 2017 Appl. Environ. Microbiol.en
dc.identifier.issn1098-5336-
dc.identifier.pmid28159784-
dc.identifier.doi10.1128/AEM.03295-16-
dc.identifier.urihttp://hdl.handle.net/10033/620840-
dc.description.abstractLegionella pneumophila cause waterborne infections resulting in severe pneumonia. High resolution genotyping of L. pneumophila isolates can be achieved by Multiple-Locus Variable number of tandem repeat Analysis (MLVA). Recently, we found that different MLVA genotypes of L. pneumophila, dominated different sites in a small drinking-water network, with a genotype-related temperature and abundance regime. The current study focuses on understanding the temperature-dependent growth kinetics of the genotypes that dominated the water network. Our aim was to model mathematically the influence of temperature on the growth kinetics of different environmental and clinical L. pneumophila genotypes and compare it with their ecological niches. Environmental strains showed a distinct temperature preference with significant differences among the growth kinetics of the three studied genotypes (Gt4, Gt6 and Gt15). Gt4 strains exhibited superior growth at lower temperatures (25-30 °C) while Gt15 strains appeared to be best adapted to relatively higher temperatures (42-45 °C). The temperature-dependent growth traits of the environmental genotypes were consistent with their distribution and temperature preferences in the water network. Clinical isolates exhibited significantly higher growth rates and reached higher maximal cell densities at 37°C and 42°C than the environmental strains. Further research on the growth preferences of L. pneumophila clinical and environmental genotypes will result in better understanding of their ecological niches in drinking water systems as well as in the human body.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTemperature-dependent growth modeling of environmental and clinical Legionella pneumophila MLVA-genotypes.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalApplied and environmental microbiologyen

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