ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620863
Title:
ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.
Authors:
Rolle, Anna-Maria; Hasenberg, Mike; Thornton, Christopher R; Solouk-Saran, Djamschid; Männ, Linda; Weski, Juliane; Maurer, Andreas; Fischer, Eliane; Spycher, Philipp R; Schibli, Roger; Boschetti, Frederic; Stegemann-Koniszewski, Sabine; Bruder, Dunja ( 0000-0003-3066-189X ) ; Severin, Gregory W; Autenrieth, Stella E; Krappmann, Sven; Davies, Genna; Pichler, Bernd J; Gunzer, Matthias; Wiehr, Stefan
Abstract:
Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo. 2016, 113 (8):E1026-33 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
23-Feb-2016
URI:
http://hdl.handle.net/10033/620863
DOI:
10.1073/pnas.1518836113
PubMed ID:
26787852
Type:
Article
Language:
en
ISSN:
1091-6490
Appears in Collections:
publications of the research group immunoregulation (IREG)

Full metadata record

DC FieldValue Language
dc.contributor.authorRolle, Anna-Mariaen
dc.contributor.authorHasenberg, Mikeen
dc.contributor.authorThornton, Christopher Ren
dc.contributor.authorSolouk-Saran, Djamschiden
dc.contributor.authorMänn, Lindaen
dc.contributor.authorWeski, Julianeen
dc.contributor.authorMaurer, Andreasen
dc.contributor.authorFischer, Elianeen
dc.contributor.authorSpycher, Philipp Ren
dc.contributor.authorSchibli, Rogeren
dc.contributor.authorBoschetti, Fredericen
dc.contributor.authorStegemann-Koniszewski, Sabineen
dc.contributor.authorBruder, Dunjaen
dc.contributor.authorSeverin, Gregory Wen
dc.contributor.authorAutenrieth, Stella Een
dc.contributor.authorKrappmann, Svenen
dc.contributor.authorDavies, Gennaen
dc.contributor.authorPichler, Bernd Jen
dc.contributor.authorGunzer, Matthiasen
dc.contributor.authorWiehr, Stefanen
dc.date.accessioned2017-03-16T09:40:13Z-
dc.date.available2017-03-16T09:40:13Z-
dc.date.issued2016-02-23-
dc.identifier.citationImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo. 2016, 113 (8):E1026-33 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490-
dc.identifier.pmid26787852-
dc.identifier.doi10.1073/pnas.1518836113-
dc.identifier.urihttp://hdl.handle.net/10033/620863-
dc.description.abstractInvasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Fungalen
dc.subject.meshAntibodies, Monoclonal, Murine-Deriveden
dc.subject.meshAspergillus fumigatusen
dc.subject.meshHumansen
dc.subject.meshMagnetic Resonance Imagingen
dc.subject.meshMiceen
dc.subject.meshPositron-Emission Tomographyen
dc.subject.meshPulmonary Aspergillosisen
dc.subject.meshRadiographyen
dc.titleImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen

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