Unravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620906
Title:
Unravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa.
Authors:
Krueger, Jonas; Pohl, Sarah; Preusse, Matthias; Kordes, Adrian; Rugen, Nils; Schniederjans, Monika; Pich, Andreas; Häussler, Susanne
Abstract:
Transcriptional regulation has a central role in cellular adaptation processes and is well investigated. In contrast, the importance of the post-transcriptional regulation on these processes is less well defined. The technological advancements have been critical to precisely quantify protein and mRNA level changes and hold promise to provide more insights into how post-transcriptional regulation determines phenotypes. In Pseudomonas aeruginosa the methyltransferase PrmC methylates peptide chain release factors to facilitate translation termination. Loss of PrmC activity abolishes anaerobic growth and leads to reduced production of quorum sensing-associated virulence factors. Here, by applying SILAC technology in combination with mRNA-sequencing, they provide evidence that the P. aeruginosa phenotype can be attributed to a change in protein to mRNA ratios of selected protein groups. The UAG-dependent translation termination was more dependent on PrmC activity than the UAA- and UGA-dependent translation termination. Additionally, a bias toward UAG stop codons in global transcriptional regulators was found. The finding that this bias in stop codon usage determines the P. aeruginosa phenotype is unexpected and adds complexity to regulatory circuits. Via modulation of PrmC activity the bacterial cell can cross-regulate targets independently of transcriptional signals, a process with an underestimated impact on the bacterial phenotype.
Affiliation:
Twincore, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynen Str. 7, 30625 Hannover, Germany.
Citation:
Unravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa. 2016, 18 (10):3583-3592 Environ. Microbiol.
Journal:
Environmental microbiology
Issue Date:
Oct-2016
URI:
http://hdl.handle.net/10033/620906
DOI:
10.1111/1462-2920.13435
PubMed ID:
27376486
Type:
Article
Language:
en
ISSN:
1462-2920
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorKrueger, Jonasen
dc.contributor.authorPohl, Sarahen
dc.contributor.authorPreusse, Matthiasen
dc.contributor.authorKordes, Adrianen
dc.contributor.authorRugen, Nilsen
dc.contributor.authorSchniederjans, Monikaen
dc.contributor.authorPich, Andreasen
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2017-05-03T14:44:40Z-
dc.date.available2017-05-03T14:44:40Z-
dc.date.issued2016-10-
dc.identifier.citationUnravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa. 2016, 18 (10):3583-3592 Environ. Microbiol.en
dc.identifier.issn1462-2920-
dc.identifier.pmid27376486-
dc.identifier.doi10.1111/1462-2920.13435-
dc.identifier.urihttp://hdl.handle.net/10033/620906-
dc.description.abstractTranscriptional regulation has a central role in cellular adaptation processes and is well investigated. In contrast, the importance of the post-transcriptional regulation on these processes is less well defined. The technological advancements have been critical to precisely quantify protein and mRNA level changes and hold promise to provide more insights into how post-transcriptional regulation determines phenotypes. In Pseudomonas aeruginosa the methyltransferase PrmC methylates peptide chain release factors to facilitate translation termination. Loss of PrmC activity abolishes anaerobic growth and leads to reduced production of quorum sensing-associated virulence factors. Here, by applying SILAC technology in combination with mRNA-sequencing, they provide evidence that the P. aeruginosa phenotype can be attributed to a change in protein to mRNA ratios of selected protein groups. The UAG-dependent translation termination was more dependent on PrmC activity than the UAA- and UGA-dependent translation termination. Additionally, a bias toward UAG stop codons in global transcriptional regulators was found. The finding that this bias in stop codon usage determines the P. aeruginosa phenotype is unexpected and adds complexity to regulatory circuits. Via modulation of PrmC activity the bacterial cell can cross-regulate targets independently of transcriptional signals, a process with an underestimated impact on the bacterial phenotype.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleUnravelling post-transcriptional PrmC-dependent regulatory mechanisms in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentTwincore, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynen Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalEnvironmental microbiologyen

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