2.50
Hdl Handle:
http://hdl.handle.net/10033/620917
Title:
Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.
Authors:
Fazli, Mustafa; Rybtke, Morten; Steiner, Elisabeth; Weidel, Elisabeth; Berthelsen, Jens; Groizeleau, Julie; Bin, Wu; Zhi, Boo Zhao; Yaming, Zhang; Kaever, Volkhard; Givskov, Michael; Hartmann, Rolf W.; Eberl, Leo; Tolker-Nielsen, Tim ( 0000-0002-9751-474X )
Abstract:
Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
Affiliation:
Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. 2017 Microbiologyopen
Journal:
MicrobiologyOpen
Issue Date:
16-Apr-2017
URI:
http://hdl.handle.net/10033/620917
DOI:
10.1002/mbo3.480
PubMed ID:
28419759
Type:
Article
Language:
en
ISSN:
2045-8827
Appears in Collections:
publications of the department drug design and optimization (HIPS]DDOP)

Full metadata record

DC FieldValue Language
dc.contributor.authorFazli, Mustafaen
dc.contributor.authorRybtke, Mortenen
dc.contributor.authorSteiner, Elisabethen
dc.contributor.authorWeidel, Elisabethen
dc.contributor.authorBerthelsen, Jensen
dc.contributor.authorGroizeleau, Julieen
dc.contributor.authorBin, Wuen
dc.contributor.authorZhi, Boo Zhaoen
dc.contributor.authorYaming, Zhangen
dc.contributor.authorKaever, Volkharden
dc.contributor.authorGivskov, Michaelen
dc.contributor.authorHartmann, Rolf W.en
dc.contributor.authorEberl, Leoen
dc.contributor.authorTolker-Nielsen, Timen
dc.date.accessioned2017-05-12T14:47:05Z-
dc.date.available2017-05-12T14:47:05Z-
dc.date.issued2017-04-16-
dc.identifier.citationRegulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. 2017 Microbiologyopenen
dc.identifier.issn2045-8827-
dc.identifier.pmid28419759-
dc.identifier.doi10.1002/mbo3.480-
dc.identifier.urihttp://hdl.handle.net/10033/620917-
dc.description.abstractKnowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilizing exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilizing exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleRegulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalMicrobiologyOpenen
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