Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620922
Title:
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort.
Authors:
Akmatov, Manas K; Koch, Nadine; Vital, Marius ( 0000-0003-4185-3475 ) ; Ahrens, Wolfgang; Flesch-Janys, Dieter; Fricke, Julia; Gatzemeier, Anja; Greiser, Halina; Günther, Kathrin; Illig, Thomas; Kaaks, Rudolf; Krone, Bastian; Kühn, Andrea; Linseisen, Jakob; Meisinger, Christine; Michels, Karin; Moebus, Susanne; Nieters, Alexandra; Obi, Nadia; Schultze, Anja; Six-Merker, Julia; Pieper, Dietmar H; Pessler, Frank
Abstract:
We examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants' satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1-V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken.
Affiliation:
TWINCORE; Zentrum für experimentelle und klinische Infectionsforsching GmbH, Feodor-Lynen Str. 17, 30625 Hannover, Germany.
Citation:
Determination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort. 2017, 7 (1):1855 Sci Rep
Journal:
Scientific reports
Issue Date:
12-May-2017
URI:
http://hdl.handle.net/10033/620922
DOI:
10.1038/s41598-017-01212-6
PubMed ID:
28500287
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group biomarker in infection and immunity [[TC] BIOM); publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorAkmatov, Manas Ken
dc.contributor.authorKoch, Nadineen
dc.contributor.authorVital, Mariusen
dc.contributor.authorAhrens, Wolfgangen
dc.contributor.authorFlesch-Janys, Dieteren
dc.contributor.authorFricke, Juliaen
dc.contributor.authorGatzemeier, Anjaen
dc.contributor.authorGreiser, Halinaen
dc.contributor.authorGünther, Kathrinen
dc.contributor.authorIllig, Thomasen
dc.contributor.authorKaaks, Rudolfen
dc.contributor.authorKrone, Bastianen
dc.contributor.authorKühn, Andreaen
dc.contributor.authorLinseisen, Jakoben
dc.contributor.authorMeisinger, Christineen
dc.contributor.authorMichels, Karinen
dc.contributor.authorMoebus, Susanneen
dc.contributor.authorNieters, Alexandraen
dc.contributor.authorObi, Nadiaen
dc.contributor.authorSchultze, Anjaen
dc.contributor.authorSix-Merker, Juliaen
dc.contributor.authorPieper, Dietmar Hen
dc.contributor.authorPessler, Franken
dc.date.accessioned2017-05-17T12:45:45Z-
dc.date.available2017-05-17T12:45:45Z-
dc.date.issued2017-05-12-
dc.identifier.citationDetermination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort. 2017, 7 (1):1855 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28500287-
dc.identifier.doi10.1038/s41598-017-01212-6-
dc.identifier.urihttp://hdl.handle.net/10033/620922-
dc.description.abstractWe examined acceptability, preference and feasibility of collecting nasal and oropharyngeal swabs, followed by microbiome analysis, in a population-based study with 524 participants. Anterior nasal and oropharyngeal swabs were collected by certified personnel. In addition, participants self-collected nasal swabs at home four weeks later. Four swab types were compared regarding (1) participants' satisfaction and acceptance and (2) detection of microbial community structures based on deep sequencing of the 16 S rRNA gene V1-V2 variable regions. All swabbing methods were highly accepted. Microbial community structure analysis revealed 846 phylotypes, 46 of which were unique to oropharynx and 164 unique to nares. The calcium alginate tipped swab was found unsuitable for microbiome determinations. Among the remaining three swab types, there were no differences in oropharyngeal microbiomes detected and only marginal differences in nasal microbiomes. Microbial community structures did not differ between staff-collected and self-collected nasal swabs. These results suggest (1) that nasal and oropharyngeal swabbing are highly feasible methods for human population-based studies that include the characterization of microbial community structures in these important ecological niches, and (2) that self-collection of nasal swabs at home can be used to reduce cost and resources needed, particularly when serial measurements are to be taken.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDetermination of nasal and oropharyngeal microbiomes in a multicenter population-based study - findings from Pretest 1 of the German National Cohort.en
dc.typeArticleen
dc.contributor.departmentTWINCORE; Zentrum für experimentelle und klinische Infectionsforsching GmbH, Feodor-Lynen Str. 17, 30625 Hannover, Germany.en
dc.identifier.journalScientific reportsen

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