Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells.

2.50
Hdl Handle:
http://hdl.handle.net/10033/620928
Title:
Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells.
Authors:
Somplatzki, Stefan; Mühlenhoff, Martina; Kröger, Andrea ( 0000-0002-3131-6580 ) ; Gerardy-Schahn, Rita; Böldicke, Thomas
Abstract:
Polysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8SiaII and ST8SiaIV. Intracellular antibodies (intrabodies; IB) expressed inside the ER and retaining proteins passing the ER such as cell surface receptors or secretory proteins provide an efficient means of protein knockdown. To inhibit the function of ST8SiaII and ST8SiaIV specific ER IBs were generated starting from two corresponding hybridoma clones. Both IBs αST8SiaII-IB and αST8SiaIV-IB were constructed in the scFv format and their functions characterized in vitro and in vivo.
Affiliation:
Helmholtz Centr for infection research
Citation:
Intrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells. 2017, 17 (1):42 BMC Biotechnol.
Journal:
BMC biotechnology
Issue Date:
12-May-2017
URI:
http://hdl.handle.net/10033/620928
DOI:
10.1186/s12896-017-0360-7
PubMed ID:
28499450
Type:
Article
Language:
en
ISSN:
1472-6750
Appears in Collections:
publications of the research group immunity and infection (IMMI); Publications of the Dept. Structure and Functions of Proteins(SFPR)

Full metadata record

DC FieldValue Language
dc.contributor.authorSomplatzki, Stefanen
dc.contributor.authorMühlenhoff, Martinaen
dc.contributor.authorKröger, Andreaen
dc.contributor.authorGerardy-Schahn, Ritaen
dc.contributor.authorBöldicke, Thomasen
dc.date.accessioned2017-05-24T12:59:17Z-
dc.date.available2017-05-24T12:59:17Z-
dc.date.issued2017-05-12-
dc.identifier.citationIntrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells. 2017, 17 (1):42 BMC Biotechnol.en
dc.identifier.issn1472-6750-
dc.identifier.pmid28499450-
dc.identifier.doi10.1186/s12896-017-0360-7-
dc.identifier.urihttp://hdl.handle.net/10033/620928-
dc.description.abstractPolysialic acid (polySia) is a carbohydrate modification of the neural cell adhesion molecule (NCAM), which is implicated in neural differentiation and plays an important role in tumor development and metastasis. Polysialylation of NCAM is mediated by two Golgi-resident polysialyltransferases (polyST) ST8SiaII and ST8SiaIV. Intracellular antibodies (intrabodies; IB) expressed inside the ER and retaining proteins passing the ER such as cell surface receptors or secretory proteins provide an efficient means of protein knockdown. To inhibit the function of ST8SiaII and ST8SiaIV specific ER IBs were generated starting from two corresponding hybridoma clones. Both IBs αST8SiaII-IB and αST8SiaIV-IB were constructed in the scFv format and their functions characterized in vitro and in vivo.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIntrabodies against the Polysialyltransferases ST8SiaII and ST8SiaIV inhibit Polysialylation of NCAM in rhabdomyosarcoma tumor cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centr for infection researchen
dc.identifier.journalBMC biotechnologyen

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