2.50
Hdl Handle:
http://hdl.handle.net/10033/620967
Title:
Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)UAA gene bldA.
Authors:
Koshla, Oksana; Lopatniuk, Maria; Rokytskyy, Ihor; Yushchuk, Oleksandr; Dacyuk, Yuriy; Fedorenko, Victor; Luzhetskyy, Andriy; Ostash, Bohdan ( 0000-0001-5904-5957 )
Abstract:
Streptomyces albus J1074 is one of the most popular and convenient hosts for heterologous expression of gene clusters directing the biosynthesis of various natural metabolic products, such as antibiotics. This fuels interest in elucidation of genetic mechanisms that may limit secondary metabolism in J1074. Here, we report the generation and initial study of J1074 mutant, deficient in gene bldA for tRNA(Leu)UAA, the only tRNA capable of decoding rare leucyl TTA codon in Streptomyces. The bldA deletion in J1074 resulted in a highly conditional Bld phenotype, with depleted formation of aerial hyphae on certain solid media. In addition, bldA mutant of J1074 was unable to produce endogenous antibacterial compounds and two heterologous antibiotics, moenomycin and aranciamycin, whose biosynthesis is directed by TTA-containing genes. We have employed a new TTA codon-specific β-galactosidase reporter system to provide genetic evidence that J1074 bldA mutant is impaired in translation of TTA. In addition, we have discussed the possible reasons for differences in the phenotypes of bldA mutants described here and in previous studies, providing knowledge to study bldA-based regulation of antibiotic biosynthesis.
Affiliation:
Helmholtz Institut für phsarmazeutische Forschung Saarland (HIPS), Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)UAA gene bldA. 2017 Arch. Microbiol.
Journal:
Archives of microbiology
Issue Date:
20-May-2017
URI:
http://hdl.handle.net/10033/620967
DOI:
10.1007/s00203-017-1389-7
PubMed ID:
28528473
Type:
Article
Language:
en
ISSN:
1432-072X
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorKoshla, Oksanaen
dc.contributor.authorLopatniuk, Mariaen
dc.contributor.authorRokytskyy, Ihoren
dc.contributor.authorYushchuk, Oleksandren
dc.contributor.authorDacyuk, Yuriyen
dc.contributor.authorFedorenko, Victoren
dc.contributor.authorLuzhetskyy, Andriyen
dc.contributor.authorOstash, Bohdanen
dc.date.accessioned2017-06-21T11:37:08Z-
dc.date.available2017-06-21T11:37:08Z-
dc.date.issued2017-05-20-
dc.identifier.citationProperties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)UAA gene bldA. 2017 Arch. Microbiol.en
dc.identifier.issn1432-072X-
dc.identifier.pmid28528473-
dc.identifier.doi10.1007/s00203-017-1389-7-
dc.identifier.urihttp://hdl.handle.net/10033/620967-
dc.description.abstractStreptomyces albus J1074 is one of the most popular and convenient hosts for heterologous expression of gene clusters directing the biosynthesis of various natural metabolic products, such as antibiotics. This fuels interest in elucidation of genetic mechanisms that may limit secondary metabolism in J1074. Here, we report the generation and initial study of J1074 mutant, deficient in gene bldA for tRNA(Leu)UAA, the only tRNA capable of decoding rare leucyl TTA codon in Streptomyces. The bldA deletion in J1074 resulted in a highly conditional Bld phenotype, with depleted formation of aerial hyphae on certain solid media. In addition, bldA mutant of J1074 was unable to produce endogenous antibacterial compounds and two heterologous antibiotics, moenomycin and aranciamycin, whose biosynthesis is directed by TTA-containing genes. We have employed a new TTA codon-specific β-galactosidase reporter system to provide genetic evidence that J1074 bldA mutant is impaired in translation of TTA. In addition, we have discussed the possible reasons for differences in the phenotypes of bldA mutants described here and in previous studies, providing knowledge to study bldA-based regulation of antibiotic biosynthesis.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleProperties of Streptomyces albus J1074 mutant deficient in tRNA(Leu)UAA gene bldA.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Institut für phsarmazeutische Forschung Saarland (HIPS), Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalArchives of microbiologyen

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