2.50
Hdl Handle:
http://hdl.handle.net/10033/620976
Title:
Variability in bacterial flagella re-growth patterns after breakage.
Authors:
Paradis, Guillaume; Chevance, Fabienne F V; Liou, Willisa; Renault, Thibaud T; Hughes, Kelly T; Rainville, Simon; Erhardt, Marc ( 0000-0001-6292-619X )
Abstract:
Many bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament. The assembly of a flagellum uses a significant proportion of the biosynthetic capacities of the cell with each filament constituting ~1% of the total cell protein. Here, we addressed a significant question whether a flagellar filament can form a new cap and resume growth after breakage. Re-growth of broken filaments was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shearing. Differential electron microscopy revealed the formation of new cap structures on broken filaments that re-grew. Flagellar filaments are therefore able to re-grow if broken by mechanical shearing forces, which are expected to occur frequently in nature. In contrast, no re-growth was observed on filaments that had been broken using ultrashort laser pulses, a technique allowing for very local damage to individual filaments. We thus conclude that assembly of a new cap at the tip of a broken filament depends on how the filament was broken.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
Variability in bacterial flagella re-growth patterns after breakage. 2017, 7 (1):1282 Sci Rep
Journal:
Scientific reports
Issue Date:
28-Apr-2017
URI:
http://hdl.handle.net/10033/620976
DOI:
10.1038/s41598-017-01302-5
PubMed ID:
28455518
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the junior research group infection biology of Salmonella (IBIS)

Full metadata record

DC FieldValue Language
dc.contributor.authorParadis, Guillaumeen
dc.contributor.authorChevance, Fabienne F Ven
dc.contributor.authorLiou, Willisaen
dc.contributor.authorRenault, Thibaud Ten
dc.contributor.authorHughes, Kelly Ten
dc.contributor.authorRainville, Simonen
dc.contributor.authorErhardt, Marcen
dc.date.accessioned2017-06-22T14:01:26Z-
dc.date.available2017-06-22T14:01:26Z-
dc.date.issued2017-04-28-
dc.identifier.citationVariability in bacterial flagella re-growth patterns after breakage. 2017, 7 (1):1282 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28455518-
dc.identifier.doi10.1038/s41598-017-01302-5-
dc.identifier.urihttp://hdl.handle.net/10033/620976-
dc.description.abstractMany bacteria swim through liquids or crawl on surfaces by rotating long appendages called flagella. Flagellar filaments are assembled from thousands of subunits that are exported through a narrow secretion channel and polymerize beneath a capping scaffold at the tip of the growing filament. The assembly of a flagellum uses a significant proportion of the biosynthetic capacities of the cell with each filament constituting ~1% of the total cell protein. Here, we addressed a significant question whether a flagellar filament can form a new cap and resume growth after breakage. Re-growth of broken filaments was visualized using sequential 3-color fluorescent labeling of filaments after mechanical shearing. Differential electron microscopy revealed the formation of new cap structures on broken filaments that re-grew. Flagellar filaments are therefore able to re-grow if broken by mechanical shearing forces, which are expected to occur frequently in nature. In contrast, no re-growth was observed on filaments that had been broken using ultrashort laser pulses, a technique allowing for very local damage to individual filaments. We thus conclude that assembly of a new cap at the tip of a broken filament depends on how the filament was broken.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleVariability in bacterial flagella re-growth patterns after breakage.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen
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