2.50
Hdl Handle:
http://hdl.handle.net/10033/620999
Title:
New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18.
Authors:
Paulus, Constanze; Rebets, Yuriy; Tokovenko, Bogdan; Nadmid, Suvd; Terekhova, Larisa P; Myronovskyi, Maksym; Zotchev, Sergey B; Rückert, Christian; Braig, Simone; Zahler, Stefan; Kalinowski, Jörn; Luzhetskyy, Andriy ( 0000-0001-6497-0047 )
Abstract:
Marine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites.
Affiliation:
Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.
Citation:
New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18. 2017, 7:42382 Sci Rep
Journal:
Scientific reports
Issue Date:
10-Feb-2017
URI:
http://hdl.handle.net/10033/620999
DOI:
10.1038/srep42382
PubMed ID:
28186197
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorPaulus, Constanzeen
dc.contributor.authorRebets, Yuriyen
dc.contributor.authorTokovenko, Bogdanen
dc.contributor.authorNadmid, Suvden
dc.contributor.authorTerekhova, Larisa Pen
dc.contributor.authorMyronovskyi, Maksymen
dc.contributor.authorZotchev, Sergey Ben
dc.contributor.authorRückert, Christianen
dc.contributor.authorBraig, Simoneen
dc.contributor.authorZahler, Stefanen
dc.contributor.authorKalinowski, Jörnen
dc.contributor.authorLuzhetskyy, Andriyen
dc.date.accessioned2017-07-05T14:47:26Z-
dc.date.available2017-07-05T14:47:26Z-
dc.date.issued2017-02-10-
dc.identifier.citationNew natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18. 2017, 7:42382 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28186197-
dc.identifier.doi10.1038/srep42382-
dc.identifier.urihttp://hdl.handle.net/10033/620999-
dc.description.abstractMarine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA and multilocus phylogenetic analysis showed that MP131-18 belongs to the genus Streptomyces. The genome of MP131-18 isolate was sequenced, and 36 gene clusters involved in the biosynthesis of 18 different types of secondary metabolites were predicted using antiSMASH analysis. The combined genomics-metabolics profiling of the strain led to the identification of several new biologically active compounds. As a result, the family of bisindole pyrroles spiroindimicins was extended with two new members, spiroindimicins E and F. Furthermore, prediction of the biosynthetic pathway for unusual α-pyrone lagunapyrone isolated from MP131-18 resulted in foresight and identification of two new compounds of this family - lagunapyrones D and E. The diversity of identified and predicted compounds from Streptomyces sp. MP131-18 demonstrates that marine-derived actinomycetes are not only a promising source of new natural products, but also represent a valuable pool of genes for combinatorial biosynthesis of secondary metabolites.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleNew natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalScientific reportsen

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