2.50
Hdl Handle:
http://hdl.handle.net/10033/621003
Title:
Cross-regulation by CrcZ RNA controls anoxic biofilm formation in Pseudomonas aeruginosa.
Authors:
Pusic, Petra; Tata, Muralidhar; Wolfinger, Michael T; Sonnleitner, Elisabeth; Häussler, Susanne; Bläsi, Udo
Abstract:
Pseudomonas aeruginosa (PA) can thrive in anaerobic biofilms in the lungs of cystic fibrosis (CF) patients. Here, we show that CrcZ is the most abundant PA14 RNA bound to the global regulator Hfq in anoxic biofilms grown in cystic fibrosis sputum medium. Hfq was crucial for anoxic biofilm formation. This observation complied with an RNAseq based transcriptome analysis and follow up studies that implicated Hfq in regulation of a central step preceding denitrification. CrcZ is known to act as a decoy that sequesters Hfq during relief of carbon catabolite repression, which in turn alleviates Hfq-mediated translational repression of catabolic genes. We therefore inferred that CrcZ indirectly impacts on biofilm formation by competing for Hfq. This hypothesis was supported by the findings that over-production of CrcZ mirrored the biofilm phenotype of the hfq deletion mutant, and that deletion of the crcZ gene augmented biofilm formation. To our knowledge, this is the first example where competition for Hfq by CrcZ cross-regulates an Hfq-dependent physiological process unrelated to carbon metabolism.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Cross-regulation by CrcZ RNA controls anoxic biofilm formation in Pseudomonas aeruginosa. 2016, 6:39621 Sci Rep
Journal:
Scientific reports
Issue Date:
21-Dec-2016
URI:
http://hdl.handle.net/10033/621003
DOI:
10.1038/srep39621
PubMed ID:
28000785
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorPusic, Petraen
dc.contributor.authorTata, Muralidharen
dc.contributor.authorWolfinger, Michael Ten
dc.contributor.authorSonnleitner, Elisabethen
dc.contributor.authorHäussler, Susanneen
dc.contributor.authorBläsi, Udoen
dc.date.accessioned2017-07-11T09:45:47Z-
dc.date.available2017-07-11T09:45:47Z-
dc.date.issued2016-12-21-
dc.identifier.citationCross-regulation by CrcZ RNA controls anoxic biofilm formation in Pseudomonas aeruginosa. 2016, 6:39621 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28000785-
dc.identifier.doi10.1038/srep39621-
dc.identifier.urihttp://hdl.handle.net/10033/621003-
dc.description.abstractPseudomonas aeruginosa (PA) can thrive in anaerobic biofilms in the lungs of cystic fibrosis (CF) patients. Here, we show that CrcZ is the most abundant PA14 RNA bound to the global regulator Hfq in anoxic biofilms grown in cystic fibrosis sputum medium. Hfq was crucial for anoxic biofilm formation. This observation complied with an RNAseq based transcriptome analysis and follow up studies that implicated Hfq in regulation of a central step preceding denitrification. CrcZ is known to act as a decoy that sequesters Hfq during relief of carbon catabolite repression, which in turn alleviates Hfq-mediated translational repression of catabolic genes. We therefore inferred that CrcZ indirectly impacts on biofilm formation by competing for Hfq. This hypothesis was supported by the findings that over-production of CrcZ mirrored the biofilm phenotype of the hfq deletion mutant, and that deletion of the crcZ gene augmented biofilm formation. To our knowledge, this is the first example where competition for Hfq by CrcZ cross-regulates an Hfq-dependent physiological process unrelated to carbon metabolism.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCross-regulation by CrcZ RNA controls anoxic biofilm formation in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen
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