2.50
Hdl Handle:
http://hdl.handle.net/10033/621010
Title:
Immune protection against reinfection with nonprimate hepacivirus.
Authors:
Pfaender, Stephanie; Walter, Stephanie; Grabski, Elena; Todt, Daniel; Bruening, Janina; Romero-Brey, Inés; Gather, Theresa; Brown, Richard J P; Hahn, Kerstin; Puff, Christina; Pfankuche, Vanessa M; Hansmann, Florian; Postel, Alexander; Becher, Paul; Thiel, Volker; Kalinke, Ulrich ( 0000-0003-0503-9564 ) ; Wagner, Bettina; Bartenschlager, Ralf; Baumgärtner, Wolfgang; Feige, Karsten; Pietschmann, Thomas ( 0000-0001-5138-6239 ) ; Cavalleri, Jessika M V; Steinmann, Eike ( 0000-0002-8771-4262 )
Abstract:
Hepatitis C virus (HCV) displays a restricted host species tropism and only humans and chimpanzees are susceptible to infection. A robust immunocompetent animal model is still lacking, hampering mechanistic analysis of virus pathogenesis, immune control, and prophylactic vaccine development. The closest homolog of HCV is the equine nonprimate hepacivirus (NPHV), which shares similar features with HCV and thus represents an animal model to study hepacivirus infections in their natural hosts. We aimed to dissect equine immune responses after experimental NPHV infection and conducted challenge experiments to investigate immune protection against secondary NPHV infections. Horses were i.v. injected with NPHV containing plasma. Flow cytometric analysis was used to monitor immune cell frequencies and activation status. All infected horses became viremic after 1 or 2 wk and viremia could be detected in two horses for several weeks followed by a delayed seroconversion and viral clearance. Histopathological examinations of liver biopsies revealed mild, periportally accentuated infiltrations of lymphocytes, macrophages, and plasma cells with some horses displaying subclinical signs of hepatitis. Following viral challenge, an activation of equine immune responses was observed. Importantly, after a primary NPHV infection, horses were protected against rechallenge with the homologous as well as a distinct isolate with only minute amounts of circulating virus being detectable.
Affiliation:
TWINCORE, Zentrum für experimentelle und klinische Infectionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
Citation:
Immune protection against reinfection with nonprimate hepacivirus. 2017, 114 (12):E2430-E2439 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
21-Mar-2017
URI:
http://hdl.handle.net/10033/621010
DOI:
10.1073/pnas.1619380114
PubMed ID:
28275093
Type:
Article
Language:
en
ISSN:
1091-6490
Appears in Collections:
publications of the department of experimental infection research ([TC] EXPI); publications of the department experimental Virology([TC]EVIR); publications of the department experimental Virology([TC]EVIR)

Full metadata record

DC FieldValue Language
dc.contributor.authorPfaender, Stephanieen
dc.contributor.authorWalter, Stephanieen
dc.contributor.authorGrabski, Elenaen
dc.contributor.authorTodt, Danielen
dc.contributor.authorBruening, Janinaen
dc.contributor.authorRomero-Brey, Inésen
dc.contributor.authorGather, Theresaen
dc.contributor.authorBrown, Richard J Pen
dc.contributor.authorHahn, Kerstinen
dc.contributor.authorPuff, Christinaen
dc.contributor.authorPfankuche, Vanessa Men
dc.contributor.authorHansmann, Florianen
dc.contributor.authorPostel, Alexanderen
dc.contributor.authorBecher, Paulen
dc.contributor.authorThiel, Volkeren
dc.contributor.authorKalinke, Ulrichen
dc.contributor.authorWagner, Bettinaen
dc.contributor.authorBartenschlager, Ralfen
dc.contributor.authorBaumgärtner, Wolfgangen
dc.contributor.authorFeige, Karstenen
dc.contributor.authorPietschmann, Thomasen
dc.contributor.authorCavalleri, Jessika M Ven
dc.contributor.authorSteinmann, Eikeen
dc.date.accessioned2017-07-13T11:52:32Z-
dc.date.available2017-07-13T11:52:32Z-
dc.date.issued2017-03-21-
dc.identifier.citationImmune protection against reinfection with nonprimate hepacivirus. 2017, 114 (12):E2430-E2439 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490-
dc.identifier.pmid28275093-
dc.identifier.doi10.1073/pnas.1619380114-
dc.identifier.urihttp://hdl.handle.net/10033/621010-
dc.description.abstractHepatitis C virus (HCV) displays a restricted host species tropism and only humans and chimpanzees are susceptible to infection. A robust immunocompetent animal model is still lacking, hampering mechanistic analysis of virus pathogenesis, immune control, and prophylactic vaccine development. The closest homolog of HCV is the equine nonprimate hepacivirus (NPHV), which shares similar features with HCV and thus represents an animal model to study hepacivirus infections in their natural hosts. We aimed to dissect equine immune responses after experimental NPHV infection and conducted challenge experiments to investigate immune protection against secondary NPHV infections. Horses were i.v. injected with NPHV containing plasma. Flow cytometric analysis was used to monitor immune cell frequencies and activation status. All infected horses became viremic after 1 or 2 wk and viremia could be detected in two horses for several weeks followed by a delayed seroconversion and viral clearance. Histopathological examinations of liver biopsies revealed mild, periportally accentuated infiltrations of lymphocytes, macrophages, and plasma cells with some horses displaying subclinical signs of hepatitis. Following viral challenge, an activation of equine immune responses was observed. Importantly, after a primary NPHV infection, horses were protected against rechallenge with the homologous as well as a distinct isolate with only minute amounts of circulating virus being detectable.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleImmune protection against reinfection with nonprimate hepacivirus.en
dc.typeArticleen
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinische Infectionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen

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