2.50
Hdl Handle:
http://hdl.handle.net/10033/621027
Title:
Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms.
Authors:
Krause, Susanne; Bremges, Andreas; Münch, Philipp C; McHardy, Alice C; Gescher, Johannes
Abstract:
This study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated by this microorganism and the ARMAN. The third archaeon in the community seems to be present in minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum. The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by the archaea of the community. Microscopic analysis revealed that growth of the ARMAN is supported by the formation of cell aggregates. These might enable feeding of the ARMAN by or on other community members.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms. 2017, 7 (1):3289 Sci Rep
Journal:
Scientific reports
Issue Date:
12-Jun-2017
URI:
http://hdl.handle.net/10033/621027
DOI:
10.1038/s41598-017-03315-6
PubMed ID:
28607432
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group bioinformatics in infection research ([BRICS] BIFO)

Full metadata record

DC FieldValue Language
dc.contributor.authorKrause, Susanneen
dc.contributor.authorBremges, Andreasen
dc.contributor.authorMünch, Philipp Cen
dc.contributor.authorMcHardy, Alice Cen
dc.contributor.authorGescher, Johannesen
dc.date.accessioned2017-08-01T13:55:16Z-
dc.date.available2017-08-01T13:55:16Z-
dc.date.issued2017-06-12-
dc.identifier.citationCharacterisation of a stable laboratory co-culture of acidophilic nanoorganisms. 2017, 7 (1):3289 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28607432-
dc.identifier.doi10.1038/s41598-017-03315-6-
dc.identifier.urihttp://hdl.handle.net/10033/621027-
dc.description.abstractThis study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated by this microorganism and the ARMAN. The third archaeon in the community seems to be present in minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum. The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by the archaea of the community. Microscopic analysis revealed that growth of the ARMAN is supported by the formation of cell aggregates. These might enable feeding of the ARMAN by or on other community members.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCharacterisation of a stable laboratory co-culture of acidophilic nanoorganisms.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen

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