A genome-wide study of two-component signal transduction systems in eight newly sequenced mutans streptococci strains

2.50
Hdl Handle:
http://hdl.handle.net/10033/621030
Title:
A genome-wide study of two-component signal transduction systems in eight newly sequenced mutans streptococci strains
Authors:
Song, Lifu; Sudhakar, Padhmanand; Wang, Wei; Conrads, Georg; Brock, Anke; Sun, Jibin; Wagner-Döbler, Irene; Zeng, An-Ping ( 0000-0001-9768-7096 )
Abstract:
Abstract Background Mutans streptococci are a group of gram-positive bacteria including the primary cariogenic dental pathogen Streptococcus mutans and closely related species. Two component systems (TCSs) composed of a signal sensing histidine kinase (HK) and a response regulator (RR) play key roles in pathogenicity, but have not been comparatively studied for these oral bacterial pathogens. Results HKs and RRs of 8 newly sequenced mutans streptococci strains, including S. sobrinus DSM20742, S. ratti DSM20564 and six S. mutans strains, were identified and compared to the TCSs of S. mutans UA159 and NN2025, two previously genome sequenced S. mutans strains. Ortholog analysis revealed 18 TCS clusters (HK-RR pairs), 2 orphan HKs and 2 orphan RRs, of which 8 TCS clusters were common to all 10 strains, 6 were absent in one or more strains, and the other 4 were exclusive to individual strains. Further classification of the predicted HKs and RRs revealed interesting aspects of their putative functions. While TCS complements were comparable within the six S. mutans strains, S. sobrinus DSM20742 lacked TCSs possibly involved in acid tolerance and fructan catabolism, and S. ratti DSM20564 possessed 3 unique TCSs but lacked the quorum-sensing related TCS (ComDE). Selected computational predictions were verified by PCR experiments. Conclusions Differences in the TCS repertoires of mutans streptococci strains, especially those of S. sobrinus and S. ratti in comparison to S. mutans, imply differences in their response mechanisms for survival in the dynamic oral environment. This genomic level study of TCSs should help in understanding the pathogenicity of these mutans streptococci strains.
Citation:
BMC Genomics. 2012 Apr 04;13(1):128
Issue Date:
4-Apr-2012
URI:
http://dx.doi.org/10.1186/1471-2164-13-128; http://hdl.handle.net/10033/621030
Type:
Journal Article
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorSong, Lifuen
dc.contributor.authorSudhakar, Padhmananden
dc.contributor.authorWang, Weien
dc.contributor.authorConrads, Georgen
dc.contributor.authorBrock, Ankeen
dc.contributor.authorSun, Jibinen
dc.contributor.authorWagner-Döbler, Ireneen
dc.contributor.authorZeng, An-Pingen
dc.date.accessioned2017-08-02T08:31:41Z-
dc.date.available2017-08-02T08:31:41Z-
dc.date.issued2012-04-04en
dc.identifier.citationBMC Genomics. 2012 Apr 04;13(1):128en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-13-128en
dc.identifier.urihttp://hdl.handle.net/10033/621030-
dc.description.abstractAbstract Background Mutans streptococci are a group of gram-positive bacteria including the primary cariogenic dental pathogen Streptococcus mutans and closely related species. Two component systems (TCSs) composed of a signal sensing histidine kinase (HK) and a response regulator (RR) play key roles in pathogenicity, but have not been comparatively studied for these oral bacterial pathogens. Results HKs and RRs of 8 newly sequenced mutans streptococci strains, including S. sobrinus DSM20742, S. ratti DSM20564 and six S. mutans strains, were identified and compared to the TCSs of S. mutans UA159 and NN2025, two previously genome sequenced S. mutans strains. Ortholog analysis revealed 18 TCS clusters (HK-RR pairs), 2 orphan HKs and 2 orphan RRs, of which 8 TCS clusters were common to all 10 strains, 6 were absent in one or more strains, and the other 4 were exclusive to individual strains. Further classification of the predicted HKs and RRs revealed interesting aspects of their putative functions. While TCS complements were comparable within the six S. mutans strains, S. sobrinus DSM20742 lacked TCSs possibly involved in acid tolerance and fructan catabolism, and S. ratti DSM20564 possessed 3 unique TCSs but lacked the quorum-sensing related TCS (ComDE). Selected computational predictions were verified by PCR experiments. Conclusions Differences in the TCS repertoires of mutans streptococci strains, especially those of S. sobrinus and S. ratti in comparison to S. mutans, imply differences in their response mechanisms for survival in the dynamic oral environment. This genomic level study of TCSs should help in understanding the pathogenicity of these mutans streptococci strains.en
dc.titleA genome-wide study of two-component signal transduction systems in eight newly sequenced mutans streptococci strainsen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderSong et al; licensee BioMed Central Ltd.en
dc.date.updated2015-09-04T08:22:52Zen
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.