High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium

2.50
Hdl Handle:
http://hdl.handle.net/10033/621031
Title:
High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium
Authors:
Yang, Yang; Biedendieck, Rebekka; Wang, Wei; Gamer, Martin; Malten, Marco; Jahn, Dieter; Deckwer, Wolf-Dieter
Abstract:
Abstract Background During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of β-lactam antibiotics were systematically improved. Results For this purpose, the PGA leader peptide was replaced by the B. megaterium LipA counterpart. A production strain deficient in the extracellular protease NprM and in xylose utilization to prevent gene inducer deprivation was constructed and employed. A buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production was developed in microscale cultivations and scaled up to a 2 Liter bioreactor. Productivities of up to 40 mg PGA per L growth medium were reached. Conclusion The combination of genetic and medium optimization led to an overall 7-fold improvement of PGA production and export in B. megaterium. The exclusion of certain amino acids from the minimal medium led for the first time to higher volumetric PGA activities than obtained for complex medium cultivations.
Citation:
Microbial Cell Factories. 2006 Nov 28;5(1):36
Issue Date:
28-Nov-2006
URI:
http://dx.doi.org/10.1186/1475-2859-5-36; http://hdl.handle.net/10033/621031
Type:
Journal Article
Appears in Collections:
Publications of Division of Biochemical Engineering (BVT)

Full metadata record

DC FieldValue Language
dc.contributor.authorYang, Yangen
dc.contributor.authorBiedendieck, Rebekkaen
dc.contributor.authorWang, Weien
dc.contributor.authorGamer, Martinen
dc.contributor.authorMalten, Marcoen
dc.contributor.authorJahn, Dieteren
dc.contributor.authorDeckwer, Wolf-Dieteren
dc.date.accessioned2017-08-02T08:47:45Z-
dc.date.available2017-08-02T08:47:45Z-
dc.date.issued2006-11-28en
dc.identifier.citationMicrobial Cell Factories. 2006 Nov 28;5(1):36en
dc.identifier.urihttp://dx.doi.org/10.1186/1475-2859-5-36en
dc.identifier.urihttp://hdl.handle.net/10033/621031-
dc.description.abstractAbstract Background During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of β-lactam antibiotics were systematically improved. Results For this purpose, the PGA leader peptide was replaced by the B. megaterium LipA counterpart. A production strain deficient in the extracellular protease NprM and in xylose utilization to prevent gene inducer deprivation was constructed and employed. A buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production was developed in microscale cultivations and scaled up to a 2 Liter bioreactor. Productivities of up to 40 mg PGA per L growth medium were reached. Conclusion The combination of genetic and medium optimization led to an overall 7-fold improvement of PGA production and export in B. megaterium. The exclusion of certain amino acids from the minimal medium led for the first time to higher volumetric PGA activities than obtained for complex medium cultivations.en
dc.titleHigh yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megateriumen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderYang et al.en
dc.date.updated2015-09-04T08:23:14Zen
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