Use of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621039
Title:
Use of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa.
Authors:
van Duuren, Jozef B J H; Müsken, Mathias; Karge, Bianka; Tomasch, Jürgen; Wittmann, Christoph; Häussler, Susanne; Brönstrup, Mark ( 0000-0002-8971-7045 )
Abstract:
Impedance spectroscopy has been applied in prokaryotic and eukaryotic cytometry as a label-free method for the investigation of adherent cells. In this paper, its use for characterizing the growth dynamics of P. aeruginosa biofilms is described and compared to crystal violet staining and confocal microscopy. The method allows monitoring the growth of biofilm-forming P. aeruginosa in a continuous and label-free manner over a period of 72 h in a 96 well plate format. Impedance curves obtained for P. aeruginosa PA14 wild type and mutant strains with a transposon insertion in pqsA and pelA genes exhibited distinct phases. We propose that the slope of the declining curve following a maximum at ca. 35-40 h is a measure of biofilm formation. Transplant experiments with P. aeruginosa biofilms and paraffin suggest that the impedance also reflects pellicle formation at the liquid-air interface, a barely considered contributor to impedance. Finally, the impairment of biofilm formation upon treatment of cultures with L-arginine and with ciprofloxacin, tobramycin and meropenem was studied by single frequency impedance spectroscopy. We suggest that these findings qualify impedance spectroscopy as an additional technique to characterize biofilm formation and its modulation by small molecule drugs.
Citation:
Use of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa. 2017, 7 (1):5223 Sci Rep
Journal:
Scientific reports
Issue Date:
12-Jul-2017
URI:
http://hdl.handle.net/10033/621039
DOI:
10.1038/s41598-017-05273-5
PubMed ID:
28701712
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
Publications of the research group Chemical Biology (CBIO); publications of the departmentment of molecular bacteriology(MOBA); collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorvan Duuren, Jozef B J Hen
dc.contributor.authorMüsken, Mathiasen
dc.contributor.authorKarge, Biankaen
dc.contributor.authorTomasch, Jürgenen
dc.contributor.authorWittmann, Christophen
dc.contributor.authorHäussler, Susanneen
dc.contributor.authorBrönstrup, Marken
dc.date.accessioned2017-08-03T14:11:48Z-
dc.date.available2017-08-03T14:11:48Z-
dc.date.issued2017-07-12-
dc.identifier.citationUse of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa. 2017, 7 (1):5223 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28701712-
dc.identifier.doi10.1038/s41598-017-05273-5-
dc.identifier.urihttp://hdl.handle.net/10033/621039-
dc.description.abstractImpedance spectroscopy has been applied in prokaryotic and eukaryotic cytometry as a label-free method for the investigation of adherent cells. In this paper, its use for characterizing the growth dynamics of P. aeruginosa biofilms is described and compared to crystal violet staining and confocal microscopy. The method allows monitoring the growth of biofilm-forming P. aeruginosa in a continuous and label-free manner over a period of 72 h in a 96 well plate format. Impedance curves obtained for P. aeruginosa PA14 wild type and mutant strains with a transposon insertion in pqsA and pelA genes exhibited distinct phases. We propose that the slope of the declining curve following a maximum at ca. 35-40 h is a measure of biofilm formation. Transplant experiments with P. aeruginosa biofilms and paraffin suggest that the impedance also reflects pellicle formation at the liquid-air interface, a barely considered contributor to impedance. Finally, the impairment of biofilm formation upon treatment of cultures with L-arginine and with ciprofloxacin, tobramycin and meropenem was studied by single frequency impedance spectroscopy. We suggest that these findings qualify impedance spectroscopy as an additional technique to characterize biofilm formation and its modulation by small molecule drugs.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleUse of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.identifier.journalScientific reportsen

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