2.50
Hdl Handle:
http://hdl.handle.net/10033/621044
Title:
Metabolic peculiarities of Aspergillus niger disclosed by comparative metabolic genomics
Authors:
Sun, Jibin; Lu, Xin; Rinas, Ursula; Ping Zeng, An
Abstract:
Abstract Background Aspergillus niger is an important industrial microorganism for the production of both metabolites, such as citric acid, and proteins, such as fungal enzymes or heterologous proteins. Despite its extensive industrial applications, the genetic inventory of this fungus is only partially understood. The recently released genome sequence opens a new horizon for both scientific studies and biotechnological applications. Results Here, we present the first genome-scale metabolic network for A. niger and an in-depth genomic comparison of this species to seven other fungi to disclose its metabolic peculiarities. The raw genomic sequences of A. niger ATCC 9029 were first annotated. The reconstructed metabolic network is based on the annotation of two A. niger genomes, CBS 513.88 and ATCC 9029, including enzymes with 988 unique EC numbers, 2,443 reactions and 2,349 metabolites. More than 1,100 enzyme-coding genes are unique to A. niger in comparison to the other seven fungi. For example, we identified additional copies of genes such as those encoding alternative mitochondrial oxidoreductase and citrate synthase in A. niger, which might contribute to the high citric acid production efficiency of this species. Moreover, nine genes were identified as encoding enzymes with EC numbers exclusively found in A. niger, mostly involved in the biosynthesis of complex secondary metabolites and degradation of aromatic compounds. Conclusion The genome-level reconstruction of the metabolic network and genome-based metabolic comparison disclose peculiarities of A. niger highly relevant to its biotechnological applications and should contribute to future rational metabolic design and systems biology studies of this black mold and related species.
Citation:
Genome Biology. 2007 Sep 04;8(9):R182
Issue Date:
4-Sep-2007
URI:
http://dx.doi.org/10.1186/gb-2007-8-9-r182; http://hdl.handle.net/10033/621044
Type:
Journal Article
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF); Publications from Division of Molekulare Struktur Biologie (MOSB)

Full metadata record

DC FieldValue Language
dc.contributor.authorSun, Jibinen
dc.contributor.authorLu, Xinen
dc.contributor.authorRinas, Ursulaen
dc.contributor.authorPing Zeng, Anen
dc.date.accessioned2017-08-04T08:46:09Z-
dc.date.available2017-08-04T08:46:09Z-
dc.date.issued2007-09-04en
dc.identifier.citationGenome Biology. 2007 Sep 04;8(9):R182en
dc.identifier.urihttp://dx.doi.org/10.1186/gb-2007-8-9-r182en
dc.identifier.urihttp://hdl.handle.net/10033/621044-
dc.description.abstractAbstract Background Aspergillus niger is an important industrial microorganism for the production of both metabolites, such as citric acid, and proteins, such as fungal enzymes or heterologous proteins. Despite its extensive industrial applications, the genetic inventory of this fungus is only partially understood. The recently released genome sequence opens a new horizon for both scientific studies and biotechnological applications. Results Here, we present the first genome-scale metabolic network for A. niger and an in-depth genomic comparison of this species to seven other fungi to disclose its metabolic peculiarities. The raw genomic sequences of A. niger ATCC 9029 were first annotated. The reconstructed metabolic network is based on the annotation of two A. niger genomes, CBS 513.88 and ATCC 9029, including enzymes with 988 unique EC numbers, 2,443 reactions and 2,349 metabolites. More than 1,100 enzyme-coding genes are unique to A. niger in comparison to the other seven fungi. For example, we identified additional copies of genes such as those encoding alternative mitochondrial oxidoreductase and citrate synthase in A. niger, which might contribute to the high citric acid production efficiency of this species. Moreover, nine genes were identified as encoding enzymes with EC numbers exclusively found in A. niger, mostly involved in the biosynthesis of complex secondary metabolites and degradation of aromatic compounds. Conclusion The genome-level reconstruction of the metabolic network and genome-based metabolic comparison disclose peculiarities of A. niger highly relevant to its biotechnological applications and should contribute to future rational metabolic design and systems biology studies of this black mold and related species.en
dc.titleMetabolic peculiarities of Aspergillus niger disclosed by comparative metabolic genomicsen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderSun et al..en
dc.date.updated2015-09-04T08:23:05Zen
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