2.50
Hdl Handle:
http://hdl.handle.net/10033/621045
Title:
Genetic tools for the investigation of Roseobacter clade bacteria
Authors:
Piekarski, Tanja; Buchholz, Ina; Drepper, Thomas; Schobert, Max; Wagner-Doebler, Irene; Tielen, Petra; Jahn, Dieter
Abstract:
Abstract Background The Roseobacter clade represents one of the most abundant, metabolically versatile and ecologically important bacterial groups found in marine habitats. A detailed molecular investigation of the regulatory and metabolic networks of these organisms is currently limited for many strains by missing suitable genetic tools. Results Conjugation and electroporation methods for the efficient and stable genetic transformation of selected Roseobacter clade bacteria including Dinoroseobacter shibae, Oceanibulbus indolifex, Phaeobacter gallaeciensis, Phaeobacter inhibens, Roseobacter denitrificans and Roseobacter litoralis were tested. For this purpose an antibiotic resistance screening was performed and suitable genetic markers were selected. Based on these transformation protocols stably maintained plasmids were identified. A plasmid encoded oxygen-independent fluorescent system was established using the flavin mononucleotide-based fluorescent protein FbFP. Finally, a chromosomal gene knockout strategy was successfully employed for the inactivation of the anaerobic metabolism regulatory gene dnr from D. shibae DFL12T. Conclusion A genetic toolbox for members of the Roseobacter clade was established. This provides a solid methodical basis for the detailed elucidation of gene regulatory and metabolic networks underlying the ecological success of this group of marine bacteria.
Citation:
BMC Microbiology. 2009 Dec 18;9(1):265
Issue Date:
18-Dec-2009
URI:
http://dx.doi.org/10.1186/1471-2180-9-265; http://hdl.handle.net/10033/621045
Type:
Journal Article
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorPiekarski, Tanjaen
dc.contributor.authorBuchholz, Inaen
dc.contributor.authorDrepper, Thomasen
dc.contributor.authorSchobert, Maxen
dc.contributor.authorWagner-Doebler, Ireneen
dc.contributor.authorTielen, Petraen
dc.contributor.authorJahn, Dieteren
dc.date.accessioned2017-08-04T09:00:47Z-
dc.date.available2017-08-04T09:00:47Z-
dc.date.issued2009-12-18en
dc.identifier.citationBMC Microbiology. 2009 Dec 18;9(1):265en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-9-265en
dc.identifier.urihttp://hdl.handle.net/10033/621045-
dc.description.abstractAbstract Background The Roseobacter clade represents one of the most abundant, metabolically versatile and ecologically important bacterial groups found in marine habitats. A detailed molecular investigation of the regulatory and metabolic networks of these organisms is currently limited for many strains by missing suitable genetic tools. Results Conjugation and electroporation methods for the efficient and stable genetic transformation of selected Roseobacter clade bacteria including Dinoroseobacter shibae, Oceanibulbus indolifex, Phaeobacter gallaeciensis, Phaeobacter inhibens, Roseobacter denitrificans and Roseobacter litoralis were tested. For this purpose an antibiotic resistance screening was performed and suitable genetic markers were selected. Based on these transformation protocols stably maintained plasmids were identified. A plasmid encoded oxygen-independent fluorescent system was established using the flavin mononucleotide-based fluorescent protein FbFP. Finally, a chromosomal gene knockout strategy was successfully employed for the inactivation of the anaerobic metabolism regulatory gene dnr from D. shibae DFL12T. Conclusion A genetic toolbox for members of the Roseobacter clade was established. This provides a solid methodical basis for the detailed elucidation of gene regulatory and metabolic networks underlying the ecological success of this group of marine bacteria.en
dc.titleGenetic tools for the investigation of Roseobacter clade bacteriaen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderPiekarski et al.en
dc.date.updated2015-09-04T08:23:02Zen
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