2.50
Hdl Handle:
http://hdl.handle.net/10033/621058
Title:
Bacterial flagella grow through an injection-diffusion mechanism.
Authors:
Renault, Thibaud T; Abraham, Anthony O; Bergmiller, Tobias; Paradis, Guillaume; Rainville, Simon; Charpentier, Emmanuelle; Guet, Călin C; Tu, Yuhai; Namba, Keiichi; Keener, James P; Minamino, Tohru; Erhardt, Marc ( 0000-0001-6292-619X )
Abstract:
The bacterial flagellum is a self-assembling nanomachine. The external flagellar filament, several times longer than a bacterial cell body, is made of a few tens of thousands subunits of a single protein: flagellin. A fundamental problem concerns the molecular mechanism of how the flagellum grows outside the cell, where no discernible energy source is available. Here, we monitored the dynamic assembly of individual flagella using in situ labelling and real-time immunostaining of elongating flagellar filaments. We report that the rate of flagellum growth, initially ∼1,700 amino acids per second, decreases with length and that the previously proposed chain mechanism does not contribute to the filament elongation dynamics. Inhibition of the proton motive force-dependent export apparatus revealed a major contribution of substrate injection in driving filament elongation. The combination of experimental and mathematical evidence demonstrates that a simple, injection-diffusion mechanism controls bacterial flagella growth outside the cell.
Citation:
Bacterial flagella grow through an injection-diffusion mechanism. 2017, 6 Elife
Journal:
eLife
Issue Date:
6-Mar-2017
URI:
http://hdl.handle.net/10033/621058
DOI:
10.7554/eLife.23136
PubMed ID:
28262091
Type:
Article
Language:
en
ISSN:
2050-084X
Appears in Collections:
publications of the junior research group infection biology of Salmonella (IBIS)

Full metadata record

DC FieldValue Language
dc.contributor.authorRenault, Thibaud Ten
dc.contributor.authorAbraham, Anthony Oen
dc.contributor.authorBergmiller, Tobiasen
dc.contributor.authorParadis, Guillaumeen
dc.contributor.authorRainville, Simonen
dc.contributor.authorCharpentier, Emmanuelleen
dc.contributor.authorGuet, Călin Cen
dc.contributor.authorTu, Yuhaien
dc.contributor.authorNamba, Keiichien
dc.contributor.authorKeener, James Pen
dc.contributor.authorMinamino, Tohruen
dc.contributor.authorErhardt, Marcen
dc.date.accessioned2017-08-16T13:12:35Z-
dc.date.available2017-08-16T13:12:35Z-
dc.date.issued2017-03-06-
dc.identifier.citationBacterial flagella grow through an injection-diffusion mechanism. 2017, 6 Elifeen
dc.identifier.issn2050-084X-
dc.identifier.pmid28262091-
dc.identifier.doi10.7554/eLife.23136-
dc.identifier.urihttp://hdl.handle.net/10033/621058-
dc.description.abstractThe bacterial flagellum is a self-assembling nanomachine. The external flagellar filament, several times longer than a bacterial cell body, is made of a few tens of thousands subunits of a single protein: flagellin. A fundamental problem concerns the molecular mechanism of how the flagellum grows outside the cell, where no discernible energy source is available. Here, we monitored the dynamic assembly of individual flagella using in situ labelling and real-time immunostaining of elongating flagellar filaments. We report that the rate of flagellum growth, initially ∼1,700 amino acids per second, decreases with length and that the previously proposed chain mechanism does not contribute to the filament elongation dynamics. Inhibition of the proton motive force-dependent export apparatus revealed a major contribution of substrate injection in driving filament elongation. The combination of experimental and mathematical evidence demonstrates that a simple, injection-diffusion mechanism controls bacterial flagella growth outside the cell.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleBacterial flagella grow through an injection-diffusion mechanism.en
dc.typeArticleen
dc.identifier.journaleLifeen

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