Cell aggregation enhances bone formation by human mesenchymal stromal cells.
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Authors
Chatterjea, ALaPointe, V L
Barradas, A
Garritsen, H
Yuan, H
Renard, A
van Blitterswijk, C A
de Beor, J
Issue Date
2017-02-15
Metadata
Show full item recordAbstract
The amount of bone generated using current tissue engineering approaches is insufficient for many clinical applications. Previous in vitro studies suggest that culturing cells as 3D aggregates can enhance their osteogenic potential, but the effect on bone formation in vivo is unknown. Here, we use agarose wells to generate uniformly sized mesenchymal stromal cell (MSC) aggregates. When combined with calcium phosphate ceramic particles and a gel prepared from human platelet-rich plasma, we generated a tissue engineered construct which significantly improved in vivo bone forming capacity as compared to the conventional system of using single cells seeded directly on the ceramic surface. Histology demonstrated the reproducibility of this system, which was tested using cells from four different donors. In vitro studies established that MSC aggregation results in an up-regulation of osteogenic transcripts. And finally, the in vivo performance of the constructs was significantly diminished when unaggregated cells were used, indicating that cell aggregation is a potent trigger of in vivo bone formation by MSCs. Cell aggregation could thus be used to improve bone tissue engineering strategies.Citation
Cell aggregation enhances bone formation by human mesenchymal stromal cells. 2017, 33:121-129 Eur Cell MaterAffiliation
Hemholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Vraunschweig, Germany.Journal
European cells & materialsPubMed ID
28198985Type
ArticleLanguage
enISSN
1473-2262ae974a485f413a2113503eed53cd6c53
10.22203/eCM.v033a09
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
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