2.50
Hdl Handle:
http://hdl.handle.net/10033/621067
Title:
Cell aggregation enhances bone formation by human mesenchymal stromal cells.
Authors:
Chatterjea, A; LaPointe, V L; Barradas, A; Garritsen, H; Yuan, H; Renard, A; van Blitterswijk, C A; de Beor, J
Abstract:
The amount of bone generated using current tissue engineering approaches is insufficient for many clinical applications. Previous in vitro studies suggest that culturing cells as 3D aggregates can enhance their osteogenic potential, but the effect on bone formation in vivo is unknown. Here, we use agarose wells to generate uniformly sized mesenchymal stromal cell (MSC) aggregates. When combined with calcium phosphate ceramic particles and a gel prepared from human platelet-rich plasma, we generated a tissue engineered construct which significantly improved in vivo bone forming capacity as compared to the conventional system of using single cells seeded directly on the ceramic surface. Histology demonstrated the reproducibility of this system, which was tested using cells from four different donors. In vitro studies established that MSC aggregation results in an up-regulation of osteogenic transcripts. And finally, the in vivo performance of the constructs was significantly diminished when unaggregated cells were used, indicating that cell aggregation is a potent trigger of in vivo bone formation by MSCs. Cell aggregation could thus be used to improve bone tissue engineering strategies.
Affiliation:
Hemholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Vraunschweig, Germany.
Citation:
Cell aggregation enhances bone formation by human mesenchymal stromal cells. 2017, 33:121-129 Eur Cell Mater
Journal:
European cells & materials
Issue Date:
15-Feb-2017
URI:
http://hdl.handle.net/10033/621067
DOI:
10.22203/eCM.v033a09
PubMed ID:
28198985
Type:
Article
Language:
en
ISSN:
1473-2262
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorChatterjea, Aen
dc.contributor.authorLaPointe, V Len
dc.contributor.authorBarradas, Aen
dc.contributor.authorGarritsen, Hen
dc.contributor.authorYuan, Hen
dc.contributor.authorRenard, Aen
dc.contributor.authorvan Blitterswijk, C Aen
dc.contributor.authorde Beor, Jen
dc.date.accessioned2017-08-21T14:05:36Z-
dc.date.available2017-08-21T14:05:36Z-
dc.date.issued2017-02-15-
dc.identifier.citationCell aggregation enhances bone formation by human mesenchymal stromal cells. 2017, 33:121-129 Eur Cell Materen
dc.identifier.issn1473-2262-
dc.identifier.pmid28198985-
dc.identifier.doi10.22203/eCM.v033a09-
dc.identifier.urihttp://hdl.handle.net/10033/621067-
dc.description.abstractThe amount of bone generated using current tissue engineering approaches is insufficient for many clinical applications. Previous in vitro studies suggest that culturing cells as 3D aggregates can enhance their osteogenic potential, but the effect on bone formation in vivo is unknown. Here, we use agarose wells to generate uniformly sized mesenchymal stromal cell (MSC) aggregates. When combined with calcium phosphate ceramic particles and a gel prepared from human platelet-rich plasma, we generated a tissue engineered construct which significantly improved in vivo bone forming capacity as compared to the conventional system of using single cells seeded directly on the ceramic surface. Histology demonstrated the reproducibility of this system, which was tested using cells from four different donors. In vitro studies established that MSC aggregation results in an up-regulation of osteogenic transcripts. And finally, the in vivo performance of the constructs was significantly diminished when unaggregated cells were used, indicating that cell aggregation is a potent trigger of in vivo bone formation by MSCs. Cell aggregation could thus be used to improve bone tissue engineering strategies.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCell aggregation enhances bone formation by human mesenchymal stromal cells.en
dc.typeArticleen
dc.contributor.departmentHemholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Vraunschweig, Germany.en
dc.identifier.journalEuropean cells & materialsen
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