Three Novel Species with Peptidoglycan Cell Walls form the New Genus Lacunisphaera gen. nov. in the Family Opitutaceae of the Verrucomicrobial Subdivision 4.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621074
Title:
Three Novel Species with Peptidoglycan Cell Walls form the New Genus Lacunisphaera gen. nov. in the Family Opitutaceae of the Verrucomicrobial Subdivision 4.
Authors:
Rast, Patrick; Glöckner, Ines; Boedeker, Christian; Jeske, Olga; Wiegand, Sandra; Reinhardt, Richard; Schumann, Peter; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Spring, Stefan; Glöckner, Frank O; Jogler, Christian; Jogler, Mareike
Abstract:
The cell wall of free-living bacteria consists of peptidoglycan (PG) and is critical for maintenance of shape as dissolved solutes cause osmotic pressure and challenge cell integrity. Surprisingly, the subdivision 4 of the phylum Verrucomicrobia appears to be exceptional in this respect. Organisms of this subdivision are described to be devoid of muramic or diaminopimelic acid (DAP), usually found as components of PG in bacterial cell walls. Here we describe three novel bacterial strains from a freshwater lake, IG15(T), IG16b(T), and IG31(T), belonging to a new genus in the subdivision 4 of Verrucomicrobia which we found to possess PG as part of their cell walls. Biochemical analysis revealed the presence of DAP not only in these novel strains, but also in Opitutus terrae PB90-1(T), the closest described relative of strains IG15(T), IG16b(T), and IG31(T). Furthermore, we found that nearly all genes necessary for peptidoglycan synthesis are present in genomes of subdivision 4 members, as well as in the complete genome sequence of strain IG16b(T). In addition, we isolated and visualized PG-sacculi for strain IG16b(T). Thus, our results challenge the concept of peptidoglycan-less free-living bacteria. Our polyphasic taxonomy approach places the novel strains in a new genus within the family Opitutaceae, for which the name Lacunisphaera gen. nov. is proposed. Strain designations for IG15(T), IG16b(T) and IG31(T) are Lacunisphaera parvula sp. nov. (=DSM 26814 = LMG 29468), L. limnophila sp. nov. (=DSM 26815 = LMG 29469) and L. anatis sp. nov. (=DSM 103142 = LMG 29578) respectively, with L. limnophila IG16b(T) being the type species of the genus.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Three Novel Species with Peptidoglycan Cell Walls form the New Genus Lacunisphaera gen. nov. in the Family Opitutaceae of the Verrucomicrobial Subdivision 4. 2017, 8:202 Front Microbiol
Journal:
Frontiers in microbiology
Issue Date:
2017
URI:
http://hdl.handle.net/10033/621074
DOI:
10.3389/fmicb.2017.00202
PubMed ID:
28243229
Type:
Article
Language:
en
ISSN:
1664-302X
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorRast, Patricken
dc.contributor.authorGlöckner, Inesen
dc.contributor.authorBoedeker, Christianen
dc.contributor.authorJeske, Olgaen
dc.contributor.authorWiegand, Sandraen
dc.contributor.authorReinhardt, Richarden
dc.contributor.authorSchumann, Peteren
dc.contributor.authorRohde, Manfreden
dc.contributor.authorSpring, Stefanen
dc.contributor.authorGlöckner, Frank Oen
dc.contributor.authorJogler, Christianen
dc.contributor.authorJogler, Mareikeen
dc.date.accessioned2017-08-23T14:23:59Z-
dc.date.available2017-08-23T14:23:59Z-
dc.date.issued2017-
dc.identifier.citationThree Novel Species with Peptidoglycan Cell Walls form the New Genus Lacunisphaera gen. nov. in the Family Opitutaceae of the Verrucomicrobial Subdivision 4. 2017, 8:202 Front Microbiolen
dc.identifier.issn1664-302X-
dc.identifier.pmid28243229-
dc.identifier.doi10.3389/fmicb.2017.00202-
dc.identifier.urihttp://hdl.handle.net/10033/621074-
dc.description.abstractThe cell wall of free-living bacteria consists of peptidoglycan (PG) and is critical for maintenance of shape as dissolved solutes cause osmotic pressure and challenge cell integrity. Surprisingly, the subdivision 4 of the phylum Verrucomicrobia appears to be exceptional in this respect. Organisms of this subdivision are described to be devoid of muramic or diaminopimelic acid (DAP), usually found as components of PG in bacterial cell walls. Here we describe three novel bacterial strains from a freshwater lake, IG15(T), IG16b(T), and IG31(T), belonging to a new genus in the subdivision 4 of Verrucomicrobia which we found to possess PG as part of their cell walls. Biochemical analysis revealed the presence of DAP not only in these novel strains, but also in Opitutus terrae PB90-1(T), the closest described relative of strains IG15(T), IG16b(T), and IG31(T). Furthermore, we found that nearly all genes necessary for peptidoglycan synthesis are present in genomes of subdivision 4 members, as well as in the complete genome sequence of strain IG16b(T). In addition, we isolated and visualized PG-sacculi for strain IG16b(T). Thus, our results challenge the concept of peptidoglycan-less free-living bacteria. Our polyphasic taxonomy approach places the novel strains in a new genus within the family Opitutaceae, for which the name Lacunisphaera gen. nov. is proposed. Strain designations for IG15(T), IG16b(T) and IG31(T) are Lacunisphaera parvula sp. nov. (=DSM 26814 = LMG 29468), L. limnophila sp. nov. (=DSM 26815 = LMG 29469) and L. anatis sp. nov. (=DSM 103142 = LMG 29578) respectively, with L. limnophila IG16b(T) being the type species of the genus.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleThree Novel Species with Peptidoglycan Cell Walls form the New Genus Lacunisphaera gen. nov. in the Family Opitutaceae of the Verrucomicrobial Subdivision 4.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalFrontiers in microbiologyen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.