2.50
Hdl Handle:
http://hdl.handle.net/10033/621088
Title:
Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales.
Authors:
Kopejtka, Karel; Tomasch, Jürgen; Zeng, Yonghui; Tichý, Martin; Sorokin, Dimitry Y; Koblížek, Michal
Abstract:
A characteristic feature of the order Rhodobacterales is the presence of a large number of photoautotrophic and photoheterotrophic species containing bacteriochlorophyll. Interestingly, these phototrophic species are phylogenetically mixed with chemotrophs. To better understand the origin of such variability, we sequenced the genomes of three closely related haloalkaliphilic species, differing in their phototrophic capacity and oxygen preference: the photoheterotrophic and facultatively anaerobic bacterium Rhodobaca barguzinensis, aerobic photoheterotroph Roseinatronobacter thiooxidans, and aerobic heterotrophic bacterium Natronohydrobacter thiooxidans. These three haloalcaliphilic species are phylogenetically related and share many common characteristics with the Rhodobacter species, forming together the Rhodobacter-Rhodobaca (RR) group. A comparative genomic analysis showed close homology of photosynthetic proteins and similarity in photosynthesis gene organization among the investigated phototrophic RR species. On the other hand, Rhodobaca barguzinensis and Roseinatronobacter thiooxidans lack an inorganic carbon fixation pathway and outer light-harvesting genes. This documents the reduction of their photosynthetic machinery towards a mostly photoheterotrophic lifestyle. Moreover, both phototrophic species contain 5-aminolevulinate synthase (encoded by the hemA gene) incorporated into their photosynthesis gene clusters, which seems to be a common feature of all aerobic anoxygenic phototrophic Alphaproteobacteria. Interestingly, the chrR-rpoE (sigma24) operon, which is part of singlet oxygen defense in phototrophic species, was found in the heterotrophic strain Natronohydrobacter thiooxidans. This suggests that this organism evolved from a photoheterotrophic ancestor through the loss of its photosynthesis genes. The overall evolution of phototrophy among the haloalkaliphilic members of the RR group is discussed.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr.7,38124 Braunschweig, Germany.
Citation:
Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales. 2017, 9 (7):1950-1962 Genome Biol Evol
Journal:
Genome biology and evolution
Issue Date:
1-Jul-2017
URI:
http://hdl.handle.net/10033/621088
DOI:
10.1093/gbe/evx141
PubMed ID:
28810712
Type:
Article
Language:
en
ISSN:
1759-6653
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorKopejtka, Karelen
dc.contributor.authorTomasch, Jürgenen
dc.contributor.authorZeng, Yonghuien
dc.contributor.authorTichý, Martinen
dc.contributor.authorSorokin, Dimitry Yen
dc.contributor.authorKoblížek, Michalen
dc.date.accessioned2017-09-04T13:22:12Z-
dc.date.available2017-09-04T13:22:12Z-
dc.date.issued2017-07-01-
dc.identifier.citationGenomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales. 2017, 9 (7):1950-1962 Genome Biol Evolen
dc.identifier.issn1759-6653-
dc.identifier.pmid28810712-
dc.identifier.doi10.1093/gbe/evx141-
dc.identifier.urihttp://hdl.handle.net/10033/621088-
dc.description.abstractA characteristic feature of the order Rhodobacterales is the presence of a large number of photoautotrophic and photoheterotrophic species containing bacteriochlorophyll. Interestingly, these phototrophic species are phylogenetically mixed with chemotrophs. To better understand the origin of such variability, we sequenced the genomes of three closely related haloalkaliphilic species, differing in their phototrophic capacity and oxygen preference: the photoheterotrophic and facultatively anaerobic bacterium Rhodobaca barguzinensis, aerobic photoheterotroph Roseinatronobacter thiooxidans, and aerobic heterotrophic bacterium Natronohydrobacter thiooxidans. These three haloalcaliphilic species are phylogenetically related and share many common characteristics with the Rhodobacter species, forming together the Rhodobacter-Rhodobaca (RR) group. A comparative genomic analysis showed close homology of photosynthetic proteins and similarity in photosynthesis gene organization among the investigated phototrophic RR species. On the other hand, Rhodobaca barguzinensis and Roseinatronobacter thiooxidans lack an inorganic carbon fixation pathway and outer light-harvesting genes. This documents the reduction of their photosynthetic machinery towards a mostly photoheterotrophic lifestyle. Moreover, both phototrophic species contain 5-aminolevulinate synthase (encoded by the hemA gene) incorporated into their photosynthesis gene clusters, which seems to be a common feature of all aerobic anoxygenic phototrophic Alphaproteobacteria. Interestingly, the chrR-rpoE (sigma24) operon, which is part of singlet oxygen defense in phototrophic species, was found in the heterotrophic strain Natronohydrobacter thiooxidans. This suggests that this organism evolved from a photoheterotrophic ancestor through the loss of its photosynthesis genes. The overall evolution of phototrophy among the haloalkaliphilic members of the RR group is discussed.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleGenomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr.7,38124 Braunschweig, Germany.en
dc.identifier.journalGenome biology and evolutionen

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