2.50
Hdl Handle:
http://hdl.handle.net/10033/621094
Title:
In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways.
Authors:
Chao, Yanjie; Li, Lei; Girodat, Dylan; Förstner, Konrad U; Said, Nelly; Corcoran, Colin; Śmiga, Michał; Papenfort, Kai; Reinhardt, Richard; Wieden, Hans-Joachim; Luisi, Ben F; Vogel, Jörg ( 0000-0003-2220-1404 )
Abstract:
Understanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators.
Affiliation:
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße 2, 97080 Würzburg, Germany.
Citation:
In Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways. 2017, 65 (1):39-51 Mol. Cell
Journal:
Molecular cell
Issue Date:
5-Jan-2017
URI:
http://hdl.handle.net/10033/621094
DOI:
10.1016/j.molcel.2016.11.002
PubMed ID:
28061332
Type:
Article
Language:
en
ISSN:
1097-4164
Appears in Collections:
publications of the Dept. of RNA biology of bacterial infections ([HIRI] RABI)

Full metadata record

DC FieldValue Language
dc.contributor.authorChao, Yanjieen
dc.contributor.authorLi, Leien
dc.contributor.authorGirodat, Dylanen
dc.contributor.authorFörstner, Konrad Uen
dc.contributor.authorSaid, Nellyen
dc.contributor.authorCorcoran, Colinen
dc.contributor.authorŚmiga, Michałen
dc.contributor.authorPapenfort, Kaien
dc.contributor.authorReinhardt, Richarden
dc.contributor.authorWieden, Hans-Joachimen
dc.contributor.authorLuisi, Ben Fen
dc.contributor.authorVogel, Jörgen
dc.date.accessioned2017-09-06T09:27:44Z-
dc.date.available2017-09-06T09:27:44Z-
dc.date.issued2017-01-05-
dc.identifier.citationIn Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways. 2017, 65 (1):39-51 Mol. Cellen
dc.identifier.issn1097-4164-
dc.identifier.pmid28061332-
dc.identifier.doi10.1016/j.molcel.2016.11.002-
dc.identifier.urihttp://hdl.handle.net/10033/621094-
dc.description.abstractUnderstanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis. Our results suggest a prominent biogenesis pathway for bacterial regulatory small RNAs whereby RNase E acts together with the RNA chaperone Hfq to liberate stable 3' fragments from various precursor RNAs. Recapitulating this process in vitro, Hfq guides RNase E cleavage of a representative small-RNA precursor for interaction with a mRNA target. In vivo, the processing is required for target regulation. Our findings reveal a general maturation mechanism for a major class of post-transcriptional regulators.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIn Vivo Cleavage Map Illuminates the Central Role of RNase E in Coding and Non-coding RNA Pathways.en
dc.typeArticleen
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße 2, 97080 Würzburg, Germany.en
dc.identifier.journalMolecular cellen

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