Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621118
Title:
Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074.
Authors:
Bilyk, Bohdan; Horbal, Liliya; Luzhetskyy, Andriy ( 0000-0001-6497-0047 )
Abstract:
Efforts to construct the Streptomyces host strain with enhanced yields of heterologous product have focussed mostly on engineering of primary metabolism and/or the deletion of endogenous biosynthetic gene clusters. However, other factors, such as chromosome compactization, have been shown to have a significant influence on gene expression levels in bacteria and fungi. The expression of genes and biosynthetic gene clusters may vary significantly depending on their location within the chromosome. Little is known about the position effect in actinomycetes, which are important producers of various industrially relevant bioactive molecules.
Affiliation:
Helmholz-Institut für pharmazeutische Forschung , Josef-Schneider-Straße2,97080 Würzburg, Germany.
Citation:
Chromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074. 2017, 16 (1):5 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
4-Jan-2017
URI:
http://hdl.handle.net/10033/621118
DOI:
10.1186/s12934-016-0619-z
PubMed ID:
28052753
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)

Full metadata record

DC FieldValue Language
dc.contributor.authorBilyk, Bohdanen
dc.contributor.authorHorbal, Liliyaen
dc.contributor.authorLuzhetskyy, Andriyen
dc.date.accessioned2017-09-25T12:13:33Z-
dc.date.available2017-09-25T12:13:33Z-
dc.date.issued2017-01-04-
dc.identifier.citationChromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074. 2017, 16 (1):5 Microb. Cell Fact.en
dc.identifier.issn1475-2859-
dc.identifier.pmid28052753-
dc.identifier.doi10.1186/s12934-016-0619-z-
dc.identifier.urihttp://hdl.handle.net/10033/621118-
dc.description.abstractEfforts to construct the Streptomyces host strain with enhanced yields of heterologous product have focussed mostly on engineering of primary metabolism and/or the deletion of endogenous biosynthetic gene clusters. However, other factors, such as chromosome compactization, have been shown to have a significant influence on gene expression levels in bacteria and fungi. The expression of genes and biosynthetic gene clusters may vary significantly depending on their location within the chromosome. Little is known about the position effect in actinomycetes, which are important producers of various industrially relevant bioactive molecules.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshChromosomes, Bacterialen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenes, Bacterialen
dc.subject.meshMultigene Familyen
dc.subject.meshStreptomycesen
dc.titleChromosomal position effect influences the heterologous expression of genes and biosynthetic gene clusters in Streptomyces albus J1074.en
dc.typeArticleen
dc.contributor.departmentHelmholz-Institut für pharmazeutische Forschung , Josef-Schneider-Straße2,97080 Würzburg, Germany.en
dc.identifier.journalMicrobial cell factoriesen

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