Bivalent mucosal peptide vaccines administered using the LCP carrier system stimulate protective immune responses against Streptococcus pyogenes infection.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621124
Title:
Bivalent mucosal peptide vaccines administered using the LCP carrier system stimulate protective immune responses against Streptococcus pyogenes infection.
Authors:
Schulze, Kai ( 0000-0003-2286-3416 ) ; Ebensen, Thomas ( 0000-0001-8906-063X ) ; Chandrudu, Saranya; Skwarczynski, Mariusz; Toth, Istvan; Olive, Colleen; Guzman, Carlos A
Abstract:
Despite the broad knowledge about the pathogenicity of Streptococcus pyogenes there is still a controversy about the correlate of protection in GAS infections. We aimed in further improving the immune responses stimulated against GAS comparing different vaccine formulations including bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and BPPCysMPEG, a derivative of the macrophage-activating lipopeptide (MALP-2), as adjuvants, respectively, to be administered with and without the universal T helper cell epitope P25 along with the optimized B cell epitope J14 of the M protein and B and T cell epitopes of SfbI. Lipopeptide based nano carrier systems (LCP) were used for efficient antigen delivery across the mucosal barrier. The stimulated immune responses were efficient in protecting mice against a respiratory challenge with a lethal dose of a heterologous S. pyogenes strain. Moreover, combination of the LCP based peptide vaccine with c-di-AMP allowed reduction of antigen dose at the same time maintaining vaccine efficacy.
Affiliation:
Helmholtz Centre for infection researchGmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Bivalent mucosal peptide vaccines administered using the LCP carrier system stimulate protective immune responses against Streptococcus pyogenes infection. 2017, 13 (8):2463-2474 Nanomedicine
Journal:
Nanomedicine : nanotechnology, biology, and medicine
Issue Date:
5-Sep-2017
URI:
http://hdl.handle.net/10033/621124
DOI:
10.1016/j.nano.2017.08.015
PubMed ID:
28887213
Type:
Article
Language:
en
ISSN:
1549-9642
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchulze, Kaien
dc.contributor.authorEbensen, Thomasen
dc.contributor.authorChandrudu, Saranyaen
dc.contributor.authorSkwarczynski, Mariuszen
dc.contributor.authorToth, Istvanen
dc.contributor.authorOlive, Colleenen
dc.contributor.authorGuzman, Carlos Aen
dc.date.accessioned2017-09-27T08:58:10Z-
dc.date.available2017-09-27T08:58:10Z-
dc.date.issued2017-09-05-
dc.identifier.citationBivalent mucosal peptide vaccines administered using the LCP carrier system stimulate protective immune responses against Streptococcus pyogenes infection. 2017, 13 (8):2463-2474 Nanomedicineen
dc.identifier.issn1549-9642-
dc.identifier.pmid28887213-
dc.identifier.doi10.1016/j.nano.2017.08.015-
dc.identifier.urihttp://hdl.handle.net/10033/621124-
dc.description.abstractDespite the broad knowledge about the pathogenicity of Streptococcus pyogenes there is still a controversy about the correlate of protection in GAS infections. We aimed in further improving the immune responses stimulated against GAS comparing different vaccine formulations including bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and BPPCysMPEG, a derivative of the macrophage-activating lipopeptide (MALP-2), as adjuvants, respectively, to be administered with and without the universal T helper cell epitope P25 along with the optimized B cell epitope J14 of the M protein and B and T cell epitopes of SfbI. Lipopeptide based nano carrier systems (LCP) were used for efficient antigen delivery across the mucosal barrier. The stimulated immune responses were efficient in protecting mice against a respiratory challenge with a lethal dose of a heterologous S. pyogenes strain. Moreover, combination of the LCP based peptide vaccine with c-di-AMP allowed reduction of antigen dose at the same time maintaining vaccine efficacy.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleBivalent mucosal peptide vaccines administered using the LCP carrier system stimulate protective immune responses against Streptococcus pyogenes infection.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection researchGmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalNanomedicine : nanotechnology, biology, and medicineen

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