2.50
Hdl Handle:
http://hdl.handle.net/10033/621125
Title:
A systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins.
Authors:
Tawk, Caroline; Sharan, Malvika; Eulalio, Ana ( 0000-0002-7355-0674 ) ; Vogel, Jörg ( 0000-0003-2220-1404 )
Abstract:
Many pathogenic bacteria utilize specialized secretion systems to deliver proteins called effectors into eukaryotic cells for manipulation of host pathways. The vast majority of known effector targets are host proteins, whereas a potential targeting of host nucleic acids remains little explored. There is only one family of effectors known to target DNA directly, and effectors binding host RNA are unknown. Here, we take a two-pronged approach to search for RNA-binding effectors, combining biocomputational prediction of RNA-binding domains (RBDs) in a newly assembled comprehensive dataset of bacterial secreted proteins, and experimental screening for RNA binding in mammalian cells. Only a small subset of effectors were predicted to carry an RBD, indicating that if RNA targeting was common, it would likely involve new types of RBDs. Our experimental evaluation of effectors with predicted RBDs further argues for a general paucity of RNA binding activities amongst bacterial effectors. We obtained evidence that PipB2 and Lpg2844, effector proteins of Salmonella and Legionella species, respectively, may harbor novel biochemical activities. Our study presenting the first systematic evaluation of the RNA-targeting potential of bacterial effectors offers a basis for discussion of whether or not host RNA is a prominent target of secreted bacterial proteins.
Affiliation:
Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße2, 97080 Würzburg, Germany.
Citation:
A systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins. 2017, 7 (1):9328 Sci Rep
Journal:
Scientific reports
Issue Date:
24-Aug-2017
URI:
http://hdl.handle.net/10033/621125
DOI:
10.1038/s41598-017-09527-0
PubMed ID:
28839189
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the Dept. of RNA biology of bacterial infections ([HIRI] RABI)

Full metadata record

DC FieldValue Language
dc.contributor.authorTawk, Carolineen
dc.contributor.authorSharan, Malvikaen
dc.contributor.authorEulalio, Anaen
dc.contributor.authorVogel, Jörgen
dc.date.accessioned2017-09-27T12:17:24Z-
dc.date.available2017-09-27T12:17:24Z-
dc.date.issued2017-08-24-
dc.identifier.citationA systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins. 2017, 7 (1):9328 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid28839189-
dc.identifier.doi10.1038/s41598-017-09527-0-
dc.identifier.urihttp://hdl.handle.net/10033/621125-
dc.description.abstractMany pathogenic bacteria utilize specialized secretion systems to deliver proteins called effectors into eukaryotic cells for manipulation of host pathways. The vast majority of known effector targets are host proteins, whereas a potential targeting of host nucleic acids remains little explored. There is only one family of effectors known to target DNA directly, and effectors binding host RNA are unknown. Here, we take a two-pronged approach to search for RNA-binding effectors, combining biocomputational prediction of RNA-binding domains (RBDs) in a newly assembled comprehensive dataset of bacterial secreted proteins, and experimental screening for RNA binding in mammalian cells. Only a small subset of effectors were predicted to carry an RBD, indicating that if RNA targeting was common, it would likely involve new types of RBDs. Our experimental evaluation of effectors with predicted RBDs further argues for a general paucity of RNA binding activities amongst bacterial effectors. We obtained evidence that PipB2 and Lpg2844, effector proteins of Salmonella and Legionella species, respectively, may harbor novel biochemical activities. Our study presenting the first systematic evaluation of the RNA-targeting potential of bacterial effectors offers a basis for discussion of whether or not host RNA is a prominent target of secreted bacterial proteins.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleA systematic analysis of the RNA-targeting potential of secreted bacterial effector proteins.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Straße2, 97080 Würzburg, Germany.en
dc.identifier.journalScientific reportsen

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