2.50
Hdl Handle:
http://hdl.handle.net/10033/621126
Title:
TCR signalling network organization at the immunological synapses of murine regulatory T cells.
Authors:
van Ham, Marco; Teich, René; Philipsen, Lars; Niemz, Jana; Amsberg, Nicole; Wissing, Josef; Nimtz, Manfred; Gröbe, Lothar; Kliche, Stefanie; Thiel, Nadine; Klawonn, Frank; Hubo, Mario; Jonuleit, Helmut; Reichardt, Peter; Müller, Andreas J ( 0000-0002-0281-6383 ) ; Huehn, Jochen ( 0000-0001-8071-1379 ) ; Jänsch, Lothar ( 0000-0002-5655-1181 )
Abstract:
Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this study identified 121 Treg cell-specific phosphorylations. Short-term triggering of T cell subsets via CD3 and CD28 widely harmonized these variations with the exception of eleven TCR signalling components that mainly regulate cytoskeleton dynamics and molecular transport. Accordingly, conjugation with B cells indeed caused variant cellular morphology and revealed a Treg cell-specific recruitment of TCR signalling components such as PKCθ, PLCγ1 and ZAP70 as well as B cell-derived CD86 into the IS. Together, results from this study support the existence of a Treg cell-specific IS and suggest Treg cell-specific cytoskeleton dynamics as a novel determinant for the unique functional properties of Treg cells.
Affiliation:
Helmholtz-Zetrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
TCR signalling network organization at the immunological synapses of murine regulatory T cells. 2017 Eur. J. Immunol.
Journal:
European journal of immunology
Issue Date:
17-Aug-2017
URI:
http://hdl.handle.net/10033/621126
DOI:
10.1002/eji.201747041
PubMed ID:
28833060
Type:
Article
Language:
en
ISSN:
1521-4141
Appears in Collections:
publications of the research group intravital microscopy in infection and immunity (INMI); publications of the division experimentelle Immunologie (EXIM); publications of the research group cellular proteom research (CPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorvan Ham, Marcoen
dc.contributor.authorTeich, Renéen
dc.contributor.authorPhilipsen, Larsen
dc.contributor.authorNiemz, Janaen
dc.contributor.authorAmsberg, Nicoleen
dc.contributor.authorWissing, Josefen
dc.contributor.authorNimtz, Manfreden
dc.contributor.authorGröbe, Lotharen
dc.contributor.authorKliche, Stefanieen
dc.contributor.authorThiel, Nadineen
dc.contributor.authorKlawonn, Franken
dc.contributor.authorHubo, Marioen
dc.contributor.authorJonuleit, Helmuten
dc.contributor.authorReichardt, Peteren
dc.contributor.authorMüller, Andreas Jen
dc.contributor.authorHuehn, Jochenen
dc.contributor.authorJänsch, Lotharen
dc.date.accessioned2017-09-28T12:14:50Z-
dc.date.available2017-09-28T12:14:50Z-
dc.date.issued2017-08-17-
dc.identifier.citationTCR signalling network organization at the immunological synapses of murine regulatory T cells. 2017 Eur. J. Immunol.en
dc.identifier.issn1521-4141-
dc.identifier.pmid28833060-
dc.identifier.doi10.1002/eji.201747041-
dc.identifier.urihttp://hdl.handle.net/10033/621126-
dc.description.abstractRegulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this study identified 121 Treg cell-specific phosphorylations. Short-term triggering of T cell subsets via CD3 and CD28 widely harmonized these variations with the exception of eleven TCR signalling components that mainly regulate cytoskeleton dynamics and molecular transport. Accordingly, conjugation with B cells indeed caused variant cellular morphology and revealed a Treg cell-specific recruitment of TCR signalling components such as PKCθ, PLCγ1 and ZAP70 as well as B cell-derived CD86 into the IS. Together, results from this study support the existence of a Treg cell-specific IS and suggest Treg cell-specific cytoskeleton dynamics as a novel determinant for the unique functional properties of Treg cells.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTCR signalling network organization at the immunological synapses of murine regulatory T cells.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zetrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalEuropean journal of immunologyen

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