Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621144
Title:
Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function.
Authors:
van der Vlugt, L E P M; Obieglo, K; Ozir-Fazalalikhan, A; Sparwasser, T ( 0000-0001-5645-902X ) ; Haeberlein, S; Smits, H H
Abstract:
Chronic schistosome infections protect against allergic airway inflammation (AAI) via the induction of IL-10-producing splenic regulatory B (Breg) cells. Previous experiments have demonstrated that schistosome-induced pulmonary B cells can also reduce AAI, but act independently of IL-10. We have now further characterized the phenotype and inhibitory activity of these protective pulmonary B cells. We excluded a role for regulatory T (Treg) cell induction as putative AAI-protective mechanisms. Schistosome-induced B cells showed increased CD86 expression and reduced cytokine expression in response to Toll-like receptor (TLR) ligands compared with control B cells. To investigate the consequences for T cell activation we cultured ovalbumin (OVA)-pulsed, schistosome-induced B cells with OVA-specific transgenic T cells and observed less Th2 cytokine expression and T cell proliferation compared with control conditions. This suppressive effect was preserved even under optimal T cell stimulation by anti-CD3/28. Blocking of the inhibitory cytokines IL-10 or TGF-β only marginally restored Th2 cytokine induction. These data suggest that schistosome-induced pulmonary B cells are impaired in their capacity to produce cytokines to TLR ligands and to induce Th2 cytokine responses independent of their antigen-presenting function. These findings underline the presence of distinct B cell subsets with different stimulatory or inhibitory properties even if induced by the same type of helminth.
Affiliation:
TwinCore, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
Citation:
Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function. 2017, 47 (9):545-554 Int. J. Parasitol.
Journal:
International journal for parasitology
Issue Date:
Aug-2017
URI:
http://hdl.handle.net/10033/621144
DOI:
10.1016/j.ijpara.2017.02.002
PubMed ID:
28385494
Type:
Article
Language:
en
ISSN:
1879-0135
Appears in Collections:
publications of the TwinCore unit Infection immunology

Full metadata record

DC FieldValue Language
dc.contributor.authorvan der Vlugt, L E P Men
dc.contributor.authorObieglo, Ken
dc.contributor.authorOzir-Fazalalikhan, Aen
dc.contributor.authorSparwasser, Ten
dc.contributor.authorHaeberlein, Sen
dc.contributor.authorSmits, H Hen
dc.date.accessioned2017-10-24T09:26:47Z-
dc.date.available2017-10-24T09:26:47Z-
dc.date.issued2017-08-
dc.identifier.citationSchistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function. 2017, 47 (9):545-554 Int. J. Parasitol.en
dc.identifier.issn1879-0135-
dc.identifier.pmid28385494-
dc.identifier.doi10.1016/j.ijpara.2017.02.002-
dc.identifier.urihttp://hdl.handle.net/10033/621144-
dc.description.abstractChronic schistosome infections protect against allergic airway inflammation (AAI) via the induction of IL-10-producing splenic regulatory B (Breg) cells. Previous experiments have demonstrated that schistosome-induced pulmonary B cells can also reduce AAI, but act independently of IL-10. We have now further characterized the phenotype and inhibitory activity of these protective pulmonary B cells. We excluded a role for regulatory T (Treg) cell induction as putative AAI-protective mechanisms. Schistosome-induced B cells showed increased CD86 expression and reduced cytokine expression in response to Toll-like receptor (TLR) ligands compared with control B cells. To investigate the consequences for T cell activation we cultured ovalbumin (OVA)-pulsed, schistosome-induced B cells with OVA-specific transgenic T cells and observed less Th2 cytokine expression and T cell proliferation compared with control conditions. This suppressive effect was preserved even under optimal T cell stimulation by anti-CD3/28. Blocking of the inhibitory cytokines IL-10 or TGF-β only marginally restored Th2 cytokine induction. These data suggest that schistosome-induced pulmonary B cells are impaired in their capacity to produce cytokines to TLR ligands and to induce Th2 cytokine responses independent of their antigen-presenting function. These findings underline the presence of distinct B cell subsets with different stimulatory or inhibitory properties even if induced by the same type of helminth.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleSchistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function.en
dc.typeArticleen
dc.contributor.departmentTwinCore, Zentrum für experimentelle und klinische Infektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalInternational journal for parasitologyen

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