2.50
Hdl Handle:
http://hdl.handle.net/10033/621152
Title:
In vitro colonisation of the distal colon by Akkermansia muciniphila is largely mucin and pH dependent.
Authors:
Van Herreweghen, F; Van den Abbeele, P; De Mulder, T; De Weirdt, R; Geirnaert, A; Hernandez-Sanabria, E; Vilchez-Vargas, R; Jauregui, R; Pieper, D H; Belzer, C; De Vos, W M; Van de Wiele, T
Abstract:
Host mucin is the main constituent of the mucus layer that covers the gut epithelium of the host, and an important source of glycans for the bacteria colonising the intestine. Akkermansia muciniphila is a mucin-degrading bacterium, abundant in the human gut, that is able to produce acetate and propionate during this degradation process. A. muciniphila has been correlated with human health in previous studies, but a mechanistic explanation is lacking. In this study, the main site of colonisation was characterised alongside additional conditions, such as differences in colon pH, prebiotic supplementation and variable mucin supply. To overcome the limitations of in vivo studies concerning variations in mucin availability and difficult access to proximal regions of the colon, a dynamic in vitro gut model (SHIME) was used. In this model, A. muciniphila was found to colonise the distal colon compartment more abundantly than the proximal colon ((±8 log copies/ml compared to ±4 log copies/ml) and the preference for the distal compartment was found to be pH-dependent. The addition of mucin caused a specific increase of A. muciniphila (±4.5 log increase over two days), far exceeding the response of other bacteria present, together with an increase in propionate. These findings suggest that colonisation and mucin degradation by A. muciniphila is dependent on pH and the concentration of mucin. Our results revealed the preference of A. muciniphila for the distal colon environment due to its higher pH and uncovered the quick and stable response of A. muciniphila to mucin supplementation.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
In vitro colonisation of the distal colon by Akkermansia muciniphila is largely mucin and pH dependent. 2017, 8 (1):81-96 Benef Microbes
Journal:
Beneficial microbes
Issue Date:
7-Feb-2017
URI:
http://hdl.handle.net/10033/621152
DOI:
10.3920/BM2016.0013
PubMed ID:
27824274
Type:
Article
Language:
en
ISSN:
1876-2891
Appears in Collections:
publications of the research group microbial interactions and processes (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorVan Herreweghen, Fen
dc.contributor.authorVan den Abbeele, Pen
dc.contributor.authorDe Mulder, Ten
dc.contributor.authorDe Weirdt, Ren
dc.contributor.authorGeirnaert, Aen
dc.contributor.authorHernandez-Sanabria, Een
dc.contributor.authorVilchez-Vargas, Ren
dc.contributor.authorJauregui, Ren
dc.contributor.authorPieper, D Hen
dc.contributor.authorBelzer, Cen
dc.contributor.authorDe Vos, W Men
dc.contributor.authorVan de Wiele, Ten
dc.date.accessioned2017-11-02T08:57:34Z-
dc.date.available2017-11-02T08:57:34Z-
dc.date.issued2017-02-07-
dc.identifier.citationIn vitro colonisation of the distal colon by Akkermansia muciniphila is largely mucin and pH dependent. 2017, 8 (1):81-96 Benef Microbesen
dc.identifier.issn1876-2891-
dc.identifier.pmid27824274-
dc.identifier.doi10.3920/BM2016.0013-
dc.identifier.urihttp://hdl.handle.net/10033/621152-
dc.description.abstractHost mucin is the main constituent of the mucus layer that covers the gut epithelium of the host, and an important source of glycans for the bacteria colonising the intestine. Akkermansia muciniphila is a mucin-degrading bacterium, abundant in the human gut, that is able to produce acetate and propionate during this degradation process. A. muciniphila has been correlated with human health in previous studies, but a mechanistic explanation is lacking. In this study, the main site of colonisation was characterised alongside additional conditions, such as differences in colon pH, prebiotic supplementation and variable mucin supply. To overcome the limitations of in vivo studies concerning variations in mucin availability and difficult access to proximal regions of the colon, a dynamic in vitro gut model (SHIME) was used. In this model, A. muciniphila was found to colonise the distal colon compartment more abundantly than the proximal colon ((±8 log copies/ml compared to ±4 log copies/ml) and the preference for the distal compartment was found to be pH-dependent. The addition of mucin caused a specific increase of A. muciniphila (±4.5 log increase over two days), far exceeding the response of other bacteria present, together with an increase in propionate. These findings suggest that colonisation and mucin degradation by A. muciniphila is dependent on pH and the concentration of mucin. Our results revealed the preference of A. muciniphila for the distal colon environment due to its higher pH and uncovered the quick and stable response of A. muciniphila to mucin supplementation.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshColonen
dc.subject.meshEpitheliumen
dc.subject.meshHumansen
dc.subject.meshHydrogen-Ion Concentrationen
dc.subject.meshModels, Biologicalen
dc.subject.meshMucinsen
dc.subject.meshPrebioticsen
dc.subject.meshVerrucomicrobiaen
dc.titleIn vitro colonisation of the distal colon by Akkermansia muciniphila is largely mucin and pH dependent.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBeneficial microbesen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.