Multivalent Siderophore-DOTAM Conjugates as Theranostics for Imaging and Treatment of Bacterial Infections.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621158
Title:
Multivalent Siderophore-DOTAM Conjugates as Theranostics for Imaging and Treatment of Bacterial Infections.
Authors:
Ferreira, Kevin; Hu, Hai-Yu; Fetz, Verena; Prochnow, Hans; Rais, Bushra; Müller, Peter P; Brönstrup, Mark ( 0000-0002-8971-7045 )
Abstract:
There is a strong need to better diagnose infections at deep body sites through noninvasive molecular imaging methods. Herein, we describe the synthesis and characterization of probes based on siderophore conjugates with catechol moieties and a central DOTAM scaffold. The probes can accommodate a metal ion as well as an antibiotic moiety and are therefore suited for theranostic purposes. The translocation of the conjugates across the outer and inner cell membranes of E. coli was confirmed by growth recovery experiments with enterobactin-deficient strains, by the antibacterial activity of ampicillin conjugates, and by confocal imaging using a fluorogen-activating protein-malachite green system adapted to E. coli. The suitability of the probes for in vivo imaging was demonstrated with a Cy5.5 conjugate in mice infected with P. aeruginosa.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
Multivalent Siderophore-DOTAM Conjugates as Theranostics for Imaging and Treatment of Bacterial Infections. 2017, 56 (28):8272-8276 Angew. Chem. Int. Ed. Engl.
Journal:
Angewandte Chemie (International ed. in English)
Issue Date:
3-Jul-2017
URI:
http://hdl.handle.net/10033/621158
DOI:
10.1002/anie.201701358
PubMed ID:
28608939
Type:
Article
Language:
en
ISSN:
1521-3773
Appears in Collections:
Publications of the research group Chemical Biology (CBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorFerreira, Kevinen
dc.contributor.authorHu, Hai-Yuen
dc.contributor.authorFetz, Verenaen
dc.contributor.authorProchnow, Hansen
dc.contributor.authorRais, Bushraen
dc.contributor.authorMüller, Peter Pen
dc.contributor.authorBrönstrup, Marken
dc.date.accessioned2017-11-03T11:55:24Z-
dc.date.available2017-11-03T11:55:24Z-
dc.date.issued2017-07-03-
dc.identifier.citationMultivalent Siderophore-DOTAM Conjugates as Theranostics for Imaging and Treatment of Bacterial Infections. 2017, 56 (28):8272-8276 Angew. Chem. Int. Ed. Engl.en
dc.identifier.issn1521-3773-
dc.identifier.pmid28608939-
dc.identifier.doi10.1002/anie.201701358-
dc.identifier.urihttp://hdl.handle.net/10033/621158-
dc.description.abstractThere is a strong need to better diagnose infections at deep body sites through noninvasive molecular imaging methods. Herein, we describe the synthesis and characterization of probes based on siderophore conjugates with catechol moieties and a central DOTAM scaffold. The probes can accommodate a metal ion as well as an antibiotic moiety and are therefore suited for theranostic purposes. The translocation of the conjugates across the outer and inner cell membranes of E. coli was confirmed by growth recovery experiments with enterobactin-deficient strains, by the antibacterial activity of ampicillin conjugates, and by confocal imaging using a fluorogen-activating protein-malachite green system adapted to E. coli. The suitability of the probes for in vivo imaging was demonstrated with a Cy5.5 conjugate in mice infected with P. aeruginosa.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleMultivalent Siderophore-DOTAM Conjugates as Theranostics for Imaging and Treatment of Bacterial Infections.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalAngewandte Chemie (International ed. in English)en

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.