2.50
Hdl Handle:
http://hdl.handle.net/10033/621169
Title:
Towards individualized diagnostics of biofilm-associated infections: a case study.
Authors:
Müsken, Mathias; Klimmek, Kathi; Sauer-Heilborn, Annette; Donnert, Monique; Sedlacek, Ludwig; Suerbaum, Sebastian; Häussler, Susanne
Abstract:
Organized within biofilm communities, bacteria exhibit resistance towards a broad spectrum of antibiotics. Thus, one might argue that bacteria isolated from biofilm-associated chronic infections should be subjected to resistance profiling under biofilm growth conditions. Various test systems have been developed to determine the biofilm-associated resistance; however, it is not clear to what extent the in vitro results reflect the situation in vivo, and whether the biofilm-resistance profile should guide clinicians in their treatment choice. To address this issue, we used confocal microscopy in combination with live/dead staining, and profiled biofilm-associated resistance of a large number (>130) of clinical Pseudomonas aeruginosa isolates from overall 15 cystic fibrosis patients. Our results demonstrate that in addition to a general non-responsiveness of bacteria when grown under biofilm conditions, there is an isolate-specific and antibiotic-specific biofilm-resistance profile. This individual resistance profile is independent on the structural properties of the biofilms. Furthermore, biofilm resistance is not linked to the resistance profile under planktonic growth conditions, or a mucoid, or small colony morphology of the tested isolates. Instead, it seems that individual biofilm structures evolve during biofilm-associated growth and are shaped by environment-specific cues. In conclusion, our results demonstrate that biofilm resistance profiles are isolate specific and cannot be deduced from commonly studied phenotypes. Further clinical studies will have to show the added value of biofilm-resistance profiling. Individualized diagnosis of biofilm resistance might lead to more rational recommendations for antimicrobial therapy and, thus, increased effectiveness of the treatment of chronically infected patients.
Affiliation:
H-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
Towards individualized diagnostics of biofilm-associated infections: a case study. 2017, 3:22 NPJ Biofilms Microbiomes
Journal:
NPJ biofilms and microbiomes
Issue Date:
2017
URI:
http://hdl.handle.net/10033/621169
DOI:
10.1038/s41522-017-0030-5
PubMed ID:
PMC5620081
PubMed Central ID:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620081/pdf/41522_2017_Article_30.pdf
Type:
Article
Language:
en
ISSN:
2055-5008
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorMüsken, Mathiasen
dc.contributor.authorKlimmek, Kathien
dc.contributor.authorSauer-Heilborn, Annetteen
dc.contributor.authorDonnert, Moniqueen
dc.contributor.authorSedlacek, Ludwigen
dc.contributor.authorSuerbaum, Sebastianen
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2017-11-08T09:06:27Z-
dc.date.available2017-11-08T09:06:27Z-
dc.date.issued2017-
dc.identifier.citationTowards individualized diagnostics of biofilm-associated infections: a case study. 2017, 3:22 NPJ Biofilms Microbiomesen
dc.identifier.issn2055-5008-
dc.identifier.pmidPMC5620081-
dc.identifier.doi10.1038/s41522-017-0030-5-
dc.identifier.urihttp://hdl.handle.net/10033/621169-
dc.description.abstractOrganized within biofilm communities, bacteria exhibit resistance towards a broad spectrum of antibiotics. Thus, one might argue that bacteria isolated from biofilm-associated chronic infections should be subjected to resistance profiling under biofilm growth conditions. Various test systems have been developed to determine the biofilm-associated resistance; however, it is not clear to what extent the in vitro results reflect the situation in vivo, and whether the biofilm-resistance profile should guide clinicians in their treatment choice. To address this issue, we used confocal microscopy in combination with live/dead staining, and profiled biofilm-associated resistance of a large number (>130) of clinical Pseudomonas aeruginosa isolates from overall 15 cystic fibrosis patients. Our results demonstrate that in addition to a general non-responsiveness of bacteria when grown under biofilm conditions, there is an isolate-specific and antibiotic-specific biofilm-resistance profile. This individual resistance profile is independent on the structural properties of the biofilms. Furthermore, biofilm resistance is not linked to the resistance profile under planktonic growth conditions, or a mucoid, or small colony morphology of the tested isolates. Instead, it seems that individual biofilm structures evolve during biofilm-associated growth and are shaped by environment-specific cues. In conclusion, our results demonstrate that biofilm resistance profiles are isolate specific and cannot be deduced from commonly studied phenotypes. Further clinical studies will have to show the added value of biofilm-resistance profiling. Individualized diagnosis of biofilm resistance might lead to more rational recommendations for antimicrobial therapy and, thus, increased effectiveness of the treatment of chronically infected patients.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleTowards individualized diagnostics of biofilm-associated infections: a case study.en
dc.typeArticleen
dc.contributor.departmentH-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalNPJ biofilms and microbiomesen
dc.identifier.pmcidhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620081/pdf/41522_2017_Article_30.pdf-
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