The transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621175
Title:
The transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner.
Authors:
Schneefeld, Marie; Busche, Tobias; Geffers, Robert; Kalinowski, Jörn; Bange, Franz-Christoph
Abstract:
The Mycobacterium tuberculosis protein encoded by the Rv1986 gene is a target for memory T cells in patients with tuberculosis, and shows strong similarities to a lysine exporter LysE of Corynebacterium glutamicum. During infection, the pathogen Mycobacterium tuberculosis adapts its metabolism to environmental changes. In this study, we found that the expression of Rv1986 is controlled by Rv1985c. Rv1985c is located directly upstream of Rv1986 with an overlapping promoter region between both genes. Semiquantitative reverse transcription PCR using an isogenic mutant of Mycobacterium tuberculosis lacking Rv1985c showed that in the presence of lysine, Rv1985c protein positively upregulated the expression of Rv1986. RNA sequencing revealed the transcription start points for both transcripts and overlapping promoters. An inverted repeat in the center of the intergenic region was identified, and binding of Rv1985c protein to the intergenic region was confirmed by electrophoretic mobility shift assays. Whole transcriptome expression analysis and RNAsequencing showed downregulated transcription of ppsBCD in the Rv1985c-mutant compared to the wild type strain. Taken together, our findings characterize the regulatory network of Rv1985c in Mycobacterium tuberculosis. Due to their similarity of an orthologous gene pair in Corynebacterium glutamicum, we suggest to rename Rv1985c to lysG(Mt), and Rv1986 to lysE(Mt).
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
The transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner. 2017, 12 (10):e0186505 PLoS ONE
Journal:
PloS one
Issue Date:
2017
URI:
http://hdl.handle.net/10033/621175
DOI:
10.1371/journal.pone.0186505
PubMed ID:
29049397
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchneefeld, Marieen
dc.contributor.authorBusche, Tobiasen
dc.contributor.authorGeffers, Roberten
dc.contributor.authorKalinowski, Jörnen
dc.contributor.authorBange, Franz-Christophen
dc.date.accessioned2017-11-14T14:08:49Z-
dc.date.available2017-11-14T14:08:49Z-
dc.date.issued2017-
dc.identifier.citationThe transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner. 2017, 12 (10):e0186505 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid29049397-
dc.identifier.doi10.1371/journal.pone.0186505-
dc.identifier.urihttp://hdl.handle.net/10033/621175-
dc.description.abstractThe Mycobacterium tuberculosis protein encoded by the Rv1986 gene is a target for memory T cells in patients with tuberculosis, and shows strong similarities to a lysine exporter LysE of Corynebacterium glutamicum. During infection, the pathogen Mycobacterium tuberculosis adapts its metabolism to environmental changes. In this study, we found that the expression of Rv1986 is controlled by Rv1985c. Rv1985c is located directly upstream of Rv1986 with an overlapping promoter region between both genes. Semiquantitative reverse transcription PCR using an isogenic mutant of Mycobacterium tuberculosis lacking Rv1985c showed that in the presence of lysine, Rv1985c protein positively upregulated the expression of Rv1986. RNA sequencing revealed the transcription start points for both transcripts and overlapping promoters. An inverted repeat in the center of the intergenic region was identified, and binding of Rv1985c protein to the intergenic region was confirmed by electrophoretic mobility shift assays. Whole transcriptome expression analysis and RNAsequencing showed downregulated transcription of ppsBCD in the Rv1985c-mutant compared to the wild type strain. Taken together, our findings characterize the regulatory network of Rv1985c in Mycobacterium tuberculosis. Due to their similarity of an orthologous gene pair in Corynebacterium glutamicum, we suggest to rename Rv1985c to lysG(Mt), and Rv1986 to lysE(Mt).en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshBacterial Proteinsen
dc.subject.meshElectrophoretic Mobility Shift Assayen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenes, Bacterialen
dc.subject.meshLysineen
dc.subject.meshMycobacterium tuberculosisen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.subject.meshSequence Homology, Amino Aciden
dc.subject.meshTrans-Activatorsen
dc.subject.meshTranscriptomeen
dc.titleThe transcriptional regulator LysG (Rv1985c) of Mycobacterium tuberculosis activates lysE (Rv1986) in a lysine-dependent manner.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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