2.50
Hdl Handle:
http://hdl.handle.net/10033/621185
Title:
The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.
Authors:
Kutle, Ivana; Sengstake, Sarah; Templin, Corinna; Glaß, Mandy; Kubsch, Tobias; Keyser, Kirsten A; Binz, Anne; Bauerfeind, Rudolf; Sodeik, Beate; Čičin-Šain, Luka; Dezeljin, Martina; Messerle, Martin
Abstract:
Cell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124Braunschweig, Germany.
Citation:
The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. 2017, 7 (1):15588 Sci Rep
Journal:
Scientific reports
Issue Date:
14-Nov-2017
URI:
http://hdl.handle.net/10033/621185
DOI:
10.1038/s41598-017-15783-x
PubMed ID:
29138436
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group immune aging and chronic infections (IMCI)

Full metadata record

DC FieldValue Language
dc.contributor.authorKutle, Ivanaen
dc.contributor.authorSengstake, Sarahen
dc.contributor.authorTemplin, Corinnaen
dc.contributor.authorGlaß, Mandyen
dc.contributor.authorKubsch, Tobiasen
dc.contributor.authorKeyser, Kirsten Aen
dc.contributor.authorBinz, Anneen
dc.contributor.authorBauerfeind, Rudolfen
dc.contributor.authorSodeik, Beateen
dc.contributor.authorČičin-Šain, Lukaen
dc.contributor.authorDezeljin, Martinaen
dc.contributor.authorMesserle, Martinen
dc.date.accessioned2017-11-28T10:16:02Z-
dc.date.available2017-11-28T10:16:02Z-
dc.date.issued2017-11-14-
dc.identifier.citationThe M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. 2017, 7 (1):15588 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid29138436-
dc.identifier.doi10.1038/s41598-017-15783-x-
dc.identifier.urihttp://hdl.handle.net/10033/621185-
dc.description.abstractCell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleThe M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124Braunschweig, Germany.en
dc.identifier.journalScientific reportsen

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