2.50
Hdl Handle:
http://hdl.handle.net/10033/621214
Title:
Determining the bacterial cell biology of Planctomycetes.
Authors:
Boedeker, Christian; Schüler, Margarete; Reintjes, Greta; Jeske, Olga; van Teeseling, Muriel C F; Jogler, Mareike; Rast, Patrick; Borchert, Daniela; Devos, Damien P; Kucklick, Martin; Schaffer, Miroslava; Kolter, Roberto; van Niftrik, Laura; Engelmann, Susanne; Amann, Rudolf; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Engelhardt, Harald; Jogler, Christian
Abstract:
Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
Determining the bacterial cell biology of Planctomycetes. 2017, 8:14853 Nat Commun
Journal:
Nature communications
Issue Date:
10-Apr-2017
URI:
http://hdl.handle.net/10033/621214
DOI:
10.1038/ncomms14853
PubMed ID:
28393831
Type:
Article
Language:
en
ISSN:
2041-1723
Appears in Collections:
publications of the central unit for microscopy (ZEIM); publications of the research group Microbial Proteomics (MPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorBoedeker, Christianen
dc.contributor.authorSchüler, Margareteen
dc.contributor.authorReintjes, Gretaen
dc.contributor.authorJeske, Olgaen
dc.contributor.authorvan Teeseling, Muriel C Fen
dc.contributor.authorJogler, Mareikeen
dc.contributor.authorRast, Patricken
dc.contributor.authorBorchert, Danielaen
dc.contributor.authorDevos, Damien Pen
dc.contributor.authorKucklick, Martinen
dc.contributor.authorSchaffer, Miroslavaen
dc.contributor.authorKolter, Robertoen
dc.contributor.authorvan Niftrik, Lauraen
dc.contributor.authorEngelmann, Susanneen
dc.contributor.authorAmann, Rudolfen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorEngelhardt, Haralden
dc.contributor.authorJogler, Christianen
dc.date.accessioned2017-12-20T14:47:25Z-
dc.date.available2017-12-20T14:47:25Z-
dc.date.issued2017-04-10-
dc.identifier.citationDetermining the bacterial cell biology of Planctomycetes. 2017, 8:14853 Nat Communen
dc.identifier.issn2041-1723-
dc.identifier.pmid28393831-
dc.identifier.doi10.1038/ncomms14853-
dc.identifier.urihttp://hdl.handle.net/10033/621214-
dc.description.abstractBacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDetermining the bacterial cell biology of Planctomycetes.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalNature communicationsen
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