2.50
Hdl Handle:
http://hdl.handle.net/10033/621241
Title:
Shaping of Intestinal Microbiota in Nlrp6- and Rag2-Deficient Mice Depends on Community Structure.
Authors:
Gálvez, Eric J C; Iljazovic, Aida; Gronow, Achim; Flavell, Richard; Strowig, Till ( 0000-0003-0185-1459 )
Abstract:
Contradicting observations have been made regarding the relative contributions of immune sensors to shaping the microbiome, yet the reasons for these discrepancies are not fully understood. Here, we investigated the contribution of environmental factors in shaping the microbiome in mice deficient in adaptive immunity (Rag2-/-) and Nlrp6, an immune sensor proposed to be involved in regulation of microbiota composition. In conventionally housed Nlrp6-/- mice, familial transmission has a significant effect on microbiota composition, complicating the analysis of genotype-dependent effects. Notably, after rederivation into standardized specific pathogen-free (SPF) conditions devoid of pathobionts, microbiota composition was indistinguishable between WT, Rag2-/-, and Nlrp6-/- mice. However, upon reintroduction of a pathobiont-containing community host, genotype-dependent differences reappear, specifically affecting the relative abundance of pathobionts such as Helicobacter spp. Our results show that the impact of Nlrp6 and also of adaptive immunity on microbiota composition depends on community structure and primarily influences pathobionts but not commensals.
Affiliation:
Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
Shaping of Intestinal Microbiota in Nlrp6- and Rag2-Deficient Mice Depends on Community Structure. 2017, 21 (13):3914-3926 Cell Rep
Journal:
Cell reports
Issue Date:
26-Dec-2017
URI:
http://hdl.handle.net/10033/621241
DOI:
10.1016/j.celrep.2017.12.027
PubMed ID:
29281837
Type:
Article
Language:
en
ISSN:
2211-1247
Appears in Collections:
publications of the research group microbial immune regulation (MIKI)

Full metadata record

DC FieldValue Language
dc.contributor.authorGálvez, Eric J Cen
dc.contributor.authorIljazovic, Aidaen
dc.contributor.authorGronow, Achimen
dc.contributor.authorFlavell, Richarden
dc.contributor.authorStrowig, Tillen
dc.date.accessioned2018-01-18T14:56:35Z-
dc.date.available2018-01-18T14:56:35Z-
dc.date.issued2017-12-26-
dc.identifier.citationShaping of Intestinal Microbiota in Nlrp6- and Rag2-Deficient Mice Depends on Community Structure. 2017, 21 (13):3914-3926 Cell Repen
dc.identifier.issn2211-1247-
dc.identifier.pmid29281837-
dc.identifier.doi10.1016/j.celrep.2017.12.027-
dc.identifier.urihttp://hdl.handle.net/10033/621241-
dc.description.abstractContradicting observations have been made regarding the relative contributions of immune sensors to shaping the microbiome, yet the reasons for these discrepancies are not fully understood. Here, we investigated the contribution of environmental factors in shaping the microbiome in mice deficient in adaptive immunity (Rag2-/-) and Nlrp6, an immune sensor proposed to be involved in regulation of microbiota composition. In conventionally housed Nlrp6-/- mice, familial transmission has a significant effect on microbiota composition, complicating the analysis of genotype-dependent effects. Notably, after rederivation into standardized specific pathogen-free (SPF) conditions devoid of pathobionts, microbiota composition was indistinguishable between WT, Rag2-/-, and Nlrp6-/- mice. However, upon reintroduction of a pathobiont-containing community host, genotype-dependent differences reappear, specifically affecting the relative abundance of pathobionts such as Helicobacter spp. Our results show that the impact of Nlrp6 and also of adaptive immunity on microbiota composition depends on community structure and primarily influences pathobionts but not commensals.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleShaping of Intestinal Microbiota in Nlrp6- and Rag2-Deficient Mice Depends on Community Structure.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalCell reportsen

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