2.50
Hdl Handle:
http://hdl.handle.net/10033/621262
Title:
Increased plasmid copy number is essential for Yersinia T3SS function and virulence.
Authors:
Wang, He; Avican, Kemal; Fahlgren, Anna; Erttmann, Saskia F; Nuss, Aaron M; Dersch, Petra ( 0000-0001-8177-3280 ) ; Fallman, Maria; Edgren, Tomas; Wolf-Watz, Hans
Abstract:
Pathogenic bacteria have evolved numerous virulence mechanisms that are essential for establishing infections. The enterobacterium Yersinia uses a type III secretion system (T3SS) encoded by a 70-kilobase, low-copy, IncFII-class virulence plasmid. We report a novel virulence strategy in Y. pseudotuberculosis in which this pathogen up-regulates the plasmid copy number during infection. We found that an increased dose of plasmid-encoded genes is indispensable for virulence and substantially elevates the expression and function of the T3SS. Remarkably, we observed direct, tight coupling between plasmid replication and T3SS function. This regulatory pathway provides a framework for further exploration of the environmental sensing mechanisms of pathogenic bacteria.
Affiliation:
Helmholtz-Zentrum für Infektionsfoschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
Increased plasmid copy number is essential for Yersinia T3SS function and virulence. 2016, 353 (6298):492-5 Science
Journal:
Science (New York, N.Y.)
Issue Date:
29-Jul-2016
URI:
http://hdl.handle.net/10033/621262
DOI:
10.1126/science.aaf7501
PubMed ID:
27365311
Type:
Article
Language:
en
ISSN:
1095-9203
Appears in Collections:
publications of the department of molecular Infectionbiology (MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Heen
dc.contributor.authorAvican, Kemalen
dc.contributor.authorFahlgren, Annaen
dc.contributor.authorErttmann, Saskia Fen
dc.contributor.authorNuss, Aaron Men
dc.contributor.authorDersch, Petraen
dc.contributor.authorFallman, Mariaen
dc.contributor.authorEdgren, Tomasen
dc.contributor.authorWolf-Watz, Hansen
dc.date.accessioned2018-02-06T09:35:50Z-
dc.date.available2018-02-06T09:35:50Z-
dc.date.issued2016-07-29-
dc.identifier.citationIncreased plasmid copy number is essential for Yersinia T3SS function and virulence. 2016, 353 (6298):492-5 Scienceen
dc.identifier.issn1095-9203-
dc.identifier.pmid27365311-
dc.identifier.doi10.1126/science.aaf7501-
dc.identifier.urihttp://hdl.handle.net/10033/621262-
dc.description.abstractPathogenic bacteria have evolved numerous virulence mechanisms that are essential for establishing infections. The enterobacterium Yersinia uses a type III secretion system (T3SS) encoded by a 70-kilobase, low-copy, IncFII-class virulence plasmid. We report a novel virulence strategy in Y. pseudotuberculosis in which this pathogen up-regulates the plasmid copy number during infection. We found that an increased dose of plasmid-encoded genes is indispensable for virulence and substantially elevates the expression and function of the T3SS. Remarkably, we observed direct, tight coupling between plasmid replication and T3SS function. This regulatory pathway provides a framework for further exploration of the environmental sensing mechanisms of pathogenic bacteria.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimalsen
dc.subject.meshGene Dosageen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshPlasmidsen
dc.subject.meshType III Secretion Systemsen
dc.subject.meshVirulenceen
dc.subject.meshVirulence Factorsen
dc.subject.meshYersinia pseudotuberculosisen
dc.subject.meshYersinia pseudotuberculosis Infectionsen
dc.titleIncreased plasmid copy number is essential for Yersinia T3SS function and virulence.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsfoschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalScience (New York, N.Y.)en

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