Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621289
Title:
Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b.
Authors:
Glaesener, Stephanie; Jaenke, Christine; Habener, Anika; Geffers, Robert ( 0000-0003-4409-016X ) ; Hagendorff, Petra; Witzlau, Katrin; Imelmann, Esther; Krueger, Andreas; Meyer-Bahlburg, Almut
Abstract:
The increased susceptibility to infections of neonates is caused by an immaturity of the immune system as a result of both qualitative and quantitative differences between neonatal and adult immune cells. With respect to B cells, neonatal antibody responses are known to be decreased. Accountable for this is an altered composition of the neonatal B cell compartment towards more immature B cells. However, it remains unclear whether the functionality of individual neonatal B cell subsets is altered as well. In the current study we therefore compared phenotypical and functional characteristics of corresponding neonatal and adult B cell subpopulations. No phenotypic differences could be identified with the exception of higher IgM expression in neonatal B cells. Functional analysis revealed differences in proliferation, survival, and B cell receptor signaling. Most importantly, neonatal B cells showed severely impaired class-switch recombination (CSR) to IgG and IgA. This was associated with increased expression of miR-181b in neonatal B cells. Deficiency of miR-181b resulted in increased CSR. With this, our results highlight intrinsic differences that contribute to weaker B cell antibody responses in newborns.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b. 2018, 13 (2):e0192230 PLoS ONE
Journal:
PloS one
Issue Date:
2018
URI:
http://hdl.handle.net/10033/621289
DOI:
10.1371/journal.pone.0192230
PubMed ID:
29389970
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorGlaesener, Stephanieen
dc.contributor.authorJaenke, Christineen
dc.contributor.authorHabener, Anikaen
dc.contributor.authorGeffers, Roberten
dc.contributor.authorHagendorff, Petraen
dc.contributor.authorWitzlau, Katrinen
dc.contributor.authorImelmann, Estheren
dc.contributor.authorKrueger, Andreasen
dc.contributor.authorMeyer-Bahlburg, Almuten
dc.date.accessioned2018-02-21T10:34:43Z-
dc.date.available2018-02-21T10:34:43Z-
dc.date.issued2018-
dc.identifier.citationDecreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b. 2018, 13 (2):e0192230 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid29389970-
dc.identifier.doi10.1371/journal.pone.0192230-
dc.identifier.urihttp://hdl.handle.net/10033/621289-
dc.description.abstractThe increased susceptibility to infections of neonates is caused by an immaturity of the immune system as a result of both qualitative and quantitative differences between neonatal and adult immune cells. With respect to B cells, neonatal antibody responses are known to be decreased. Accountable for this is an altered composition of the neonatal B cell compartment towards more immature B cells. However, it remains unclear whether the functionality of individual neonatal B cell subsets is altered as well. In the current study we therefore compared phenotypical and functional characteristics of corresponding neonatal and adult B cell subpopulations. No phenotypic differences could be identified with the exception of higher IgM expression in neonatal B cells. Functional analysis revealed differences in proliferation, survival, and B cell receptor signaling. Most importantly, neonatal B cells showed severely impaired class-switch recombination (CSR) to IgG and IgA. This was associated with increased expression of miR-181b in neonatal B cells. Deficiency of miR-181b resulted in increased CSR. With this, our results highlight intrinsic differences that contribute to weaker B cell antibody responses in newborns.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDecreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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