Polymicrobial sepsis and non-specific immunization induce adaptive immunosuppression to a similar degree.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621290
Title:
Polymicrobial sepsis and non-specific immunization induce adaptive immunosuppression to a similar degree.
Authors:
Schmoeckel, Katrin; Mrochen, Daniel M; Hühn, Jochen; Pötschke, Christian; Bröker, Barbara M
Abstract:
Sepsis is frequently complicated by a state of profound immunosuppression, in its extreme form known as immunoparalysis. We have studied the role of the adaptive immune system in the murine acute peritonitis model. To read out adaptive immunosuppression, we primed post-septic and control animals by immunization with the model antigen TNP-ovalbumin in alum, and measured the specific antibody-responses via ELISA and ELISpot assay as well as T-cell responses in a proliferation assay after restimulation. Specific antibody titers, antibody affinity and plasma cell counts in the bone marrow were reduced in post-septic animals. The antigen-induced splenic proliferation was also impaired. The adaptive immunosuppression was positively correlated with an overwhelming general antibody response to the septic insult. Remarkably, antigen "overload" by non-specific immunization induced a similar degree of adaptive immunosuppression in the absence of sepsis. In both settings, depletion of regulatory T cells before priming reversed some parameters of the immunosuppression. In conclusion, our data show that adaptive immunosuppression occurs independent of profound systemic inflammation and life-threatening illness.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Polymicrobial sepsis and non-specific immunization induce adaptive immunosuppression to a similar degree. 2018, 13 (2):e0192197 PLoS ONE
Journal:
PloS one
Issue Date:
2018
URI:
http://hdl.handle.net/10033/621290
DOI:
10.1371/journal.pone.0192197
PubMed ID:
29415028
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the division experimentelle Immunologie (EXIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchmoeckel, Katrinen
dc.contributor.authorMrochen, Daniel Men
dc.contributor.authorHühn, Jochenen
dc.contributor.authorPötschke, Christianen
dc.contributor.authorBröker, Barbara Men
dc.date.accessioned2018-02-21T11:53:15Z-
dc.date.available2018-02-21T11:53:15Z-
dc.date.issued2018-
dc.identifier.citationPolymicrobial sepsis and non-specific immunization induce adaptive immunosuppression to a similar degree. 2018, 13 (2):e0192197 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid29415028-
dc.identifier.doi10.1371/journal.pone.0192197-
dc.identifier.urihttp://hdl.handle.net/10033/621290-
dc.description.abstractSepsis is frequently complicated by a state of profound immunosuppression, in its extreme form known as immunoparalysis. We have studied the role of the adaptive immune system in the murine acute peritonitis model. To read out adaptive immunosuppression, we primed post-septic and control animals by immunization with the model antigen TNP-ovalbumin in alum, and measured the specific antibody-responses via ELISA and ELISpot assay as well as T-cell responses in a proliferation assay after restimulation. Specific antibody titers, antibody affinity and plasma cell counts in the bone marrow were reduced in post-septic animals. The antigen-induced splenic proliferation was also impaired. The adaptive immunosuppression was positively correlated with an overwhelming general antibody response to the septic insult. Remarkably, antigen "overload" by non-specific immunization induced a similar degree of adaptive immunosuppression in the absence of sepsis. In both settings, depletion of regulatory T cells before priming reversed some parameters of the immunosuppression. In conclusion, our data show that adaptive immunosuppression occurs independent of profound systemic inflammation and life-threatening illness.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titlePolymicrobial sepsis and non-specific immunization induce adaptive immunosuppression to a similar degree.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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