Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621297
Title:
Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum.
Authors:
Spring, Stefan; Bunk, Boyke; Spröer, Cathrin; Schumann, Peter; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Tindall, Brian J; Klenk, Hans-Peter
Abstract:
The recently isolated strain L21-Fru-ABTrepresents moderately halophilic, obligately anaerobic and saccharolytic bacteria that thrive in the suboxic transition zones of hypersaline microbial mats. Phylogenetic analyses based on 16S rRNA genes, RpoB proteins and gene content indicated that strain L21-Fru-ABTrepresents a novel species and genus affiliated with a distinct phylum-level lineage originally designated Verrucomicrobia subdivision 5. A survey of environmental 16S rRNA gene sequences revealed that members of this newly recognized phylum are wide-spread and ecologically important in various anoxic environments ranging from hypersaline sediments to wastewater and the intestine of animals. Characteristic phenotypic traits of the novel strain included the formation of extracellular polymeric substances, a Gram-negative cell wall containing peptidoglycan and the absence of odd-numbered cellular fatty acids. Unusual metabolic features deduced from analysis of the genome sequence were the production of sucrose as osmoprotectant, an atypical glycolytic pathway lacking pyruvate kinase and the synthesis of isoprenoids via mevalonate. On the basis of the analyses of phenotypic, genomic and environmental data, it is proposed that strain L21-Fru-ABTand related bacteria are specifically adapted to the utilization of sulfated glycopolymers produced in microbial mats or biofilms.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum. 2016, 10 (12):2801-2816 ISME J
Journal:
The ISME journal
Issue Date:
2016
URI:
http://hdl.handle.net/10033/621297
DOI:
10.1038/ismej.2016.84
PubMed ID:
27300277
Type:
Article
Language:
en
ISSN:
1751-7370
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorSpring, Stefanen
dc.contributor.authorBunk, Boykeen
dc.contributor.authorSpröer, Cathrinen
dc.contributor.authorSchumann, Peteren
dc.contributor.authorRohde, Manfreden
dc.contributor.authorTindall, Brian Jen
dc.contributor.authorKlenk, Hans-Peteren
dc.date.accessioned2018-02-26T10:15:23Z-
dc.date.available2018-02-26T10:15:23Z-
dc.date.issued2016-
dc.identifier.citationCharacterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum. 2016, 10 (12):2801-2816 ISME Jen
dc.identifier.issn1751-7370-
dc.identifier.pmid27300277-
dc.identifier.doi10.1038/ismej.2016.84-
dc.identifier.urihttp://hdl.handle.net/10033/621297-
dc.description.abstractThe recently isolated strain L21-Fru-ABTrepresents moderately halophilic, obligately anaerobic and saccharolytic bacteria that thrive in the suboxic transition zones of hypersaline microbial mats. Phylogenetic analyses based on 16S rRNA genes, RpoB proteins and gene content indicated that strain L21-Fru-ABTrepresents a novel species and genus affiliated with a distinct phylum-level lineage originally designated Verrucomicrobia subdivision 5. A survey of environmental 16S rRNA gene sequences revealed that members of this newly recognized phylum are wide-spread and ecologically important in various anoxic environments ranging from hypersaline sediments to wastewater and the intestine of animals. Characteristic phenotypic traits of the novel strain included the formation of extracellular polymeric substances, a Gram-negative cell wall containing peptidoglycan and the absence of odd-numbered cellular fatty acids. Unusual metabolic features deduced from analysis of the genome sequence were the production of sucrose as osmoprotectant, an atypical glycolytic pathway lacking pyruvate kinase and the synthesis of isoprenoids via mevalonate. On the basis of the analyses of phenotypic, genomic and environmental data, it is proposed that strain L21-Fru-ABTand related bacteria are specifically adapted to the utilization of sulfated glycopolymers produced in microbial mats or biofilms.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshBacterial Proteinsen
dc.subject.meshBacterial Typing Techniquesen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Ribosomalen
dc.subject.meshFatty Acidsen
dc.subject.meshLakesen
dc.subject.meshPhylogenyen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshVerrucomicrobiaen
dc.titleCharacterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalThe ISME journalen
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