2.50
Hdl Handle:
http://hdl.handle.net/10033/621309
Title:
Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins.
Authors:
Romero Pastrana, Francisco; Neef, Jolanda; Koedijk, Dennis G A M; de Graaf, Douwe; Duipmans, José; Jonkman, Marcel F; Engelmann, Susanne; van Dijl, Jan Maarten; Buist, Girbe
Abstract:
Human antibody responses to pathogens, like Staphylococcus aureus, are important indicators for in vivo expression and immunogenicity of particular bacterial components. Accordingly, comparing the antibody responses to S. aureus components may serve to predict their potential applicability as antigens for vaccination. The present study was aimed at assessing immunoglobulin G (IgG) responses elicited by non-covalently cell surface-bound proteins of S. aureus, which thus far received relatively little attention. To this end, we applied plasma samples from patients with the genetic blistering disease epidermolysis bullosa (EB) and healthy S. aureus carriers. Of note, wounds of EB patients are highly colonized with S. aureus and accordingly these patients are more seriously exposed to staphylococcal antigens than healthy individuals. Ten non-covalently cell surface-bound proteins of S. aureus, namely Atl, Eap, Efb, EMP, IsaA, LukG, LukH, SA0710, Sle1 and SsaA2, were selected by bioinformatics and biochemical approaches. These antigens were recombinantly expressed, purified and tested for specific IgG responses using human plasma. We show that high exposure of EB patients to S. aureus is mirrored by elevated IgG levels against all tested non-covalently cell wall-bound staphylococcal antigens. This implies that these S. aureus cell surface proteins are prime targets for the human immune system.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins. 2018, 8 (1):3234 Sci Rep
Journal:
Scientific reports
Issue Date:
19-Feb-2018
URI:
http://hdl.handle.net/10033/621309
DOI:
10.1038/s41598-018-21724-z
PubMed ID:
29459694
Type:
Article
Language:
en
ISSN:
2045-2322
Appears in Collections:
publications of the research group Microbial Proteomics (MPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorRomero Pastrana, Franciscoen
dc.contributor.authorNeef, Jolandaen
dc.contributor.authorKoedijk, Dennis G A Men
dc.contributor.authorde Graaf, Douween
dc.contributor.authorDuipmans, Joséen
dc.contributor.authorJonkman, Marcel Fen
dc.contributor.authorEngelmann, Susanneen
dc.contributor.authorvan Dijl, Jan Maartenen
dc.contributor.authorBuist, Girbeen
dc.date.accessioned2018-03-07T14:38:45Z-
dc.date.available2018-03-07T14:38:45Z-
dc.date.issued2018-02-19-
dc.identifier.citationHuman antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins. 2018, 8 (1):3234 Sci Repen
dc.identifier.issn2045-2322-
dc.identifier.pmid29459694-
dc.identifier.doi10.1038/s41598-018-21724-z-
dc.identifier.urihttp://hdl.handle.net/10033/621309-
dc.description.abstractHuman antibody responses to pathogens, like Staphylococcus aureus, are important indicators for in vivo expression and immunogenicity of particular bacterial components. Accordingly, comparing the antibody responses to S. aureus components may serve to predict their potential applicability as antigens for vaccination. The present study was aimed at assessing immunoglobulin G (IgG) responses elicited by non-covalently cell surface-bound proteins of S. aureus, which thus far received relatively little attention. To this end, we applied plasma samples from patients with the genetic blistering disease epidermolysis bullosa (EB) and healthy S. aureus carriers. Of note, wounds of EB patients are highly colonized with S. aureus and accordingly these patients are more seriously exposed to staphylococcal antigens than healthy individuals. Ten non-covalently cell surface-bound proteins of S. aureus, namely Atl, Eap, Efb, EMP, IsaA, LukG, LukH, SA0710, Sle1 and SsaA2, were selected by bioinformatics and biochemical approaches. These antigens were recombinantly expressed, purified and tested for specific IgG responses using human plasma. We show that high exposure of EB patients to S. aureus is mirrored by elevated IgG levels against all tested non-covalently cell wall-bound staphylococcal antigens. This implies that these S. aureus cell surface proteins are prime targets for the human immune system.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleHuman antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalScientific reportsen

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