2.50
Hdl Handle:
http://hdl.handle.net/10033/621313
Title:
Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections.
Authors:
Castoldi, Arianna; Herr, Christian; Niederstraßer, Julia; Labouta, Hagar Ibrahim; Melero, Ana; Gordon, Sarah; Schneider-Daum, Nicole; Bals, Robert; Lehr, Claus Michael ( 0000-0002-5864-8462 )
Abstract:
The influence of vitamin D3 and its metabolites calcifediol (25(OH)D) and calcitriol on immune regulation and inflammation is well described, and raises the question of potential benefit against bacterial infections. In the current study, 25(OH)D was encapsulated in liposomes to enable aerosolisation, and tested for the ability to prevent pulmonary infection by Pseudomonas aeruginosa. Prepared 25(OH)D-loaded liposomes were nanosized and monodisperse, with a negative surface charge and a 25(OH)D entrapment efficiency of approximately 23%. Jet nebulisation of liposomes was seen to yield an aerosol suitable for tracheo-bronchial deposition. Interestingly, 25(OH)D in either liposomes or ethanolic solution had no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells (LA-4); treatment of infected, human bronchial 16-HBE cells with 25(OH)D liposomes however resulted in a significant reduction in bacterial survival. Together with the importance of selecting an application-appropriate in vitro model, the current study illustrates the feasibility and practicality of employing liposomes as a means to achieve 25(OH)D lung deposition. 25(OH)D-loaded liposomes further demonstrated promising effects regarding prevention of Pseudomonas infection in human bronchial epithelial cells.
Affiliation:
HIPS, Helmholtz-Institut für pharmazeutische Forschung Saarland, Universitätscampus 8.1, 66123 Saarbrücken, Germany.
Citation:
Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. 2017, 118:62-67 Eur J Pharm Biopharm
Journal:
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Issue Date:
Sep-2017
URI:
http://hdl.handle.net/10033/621313
DOI:
10.1016/j.ejpb.2016.11.026
PubMed ID:
27888144
Type:
Article
Language:
en
ISSN:
1873-3441
Appears in Collections:
publications of the department drug delivery ([TC] DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorCastoldi, Ariannaen
dc.contributor.authorHerr, Christianen
dc.contributor.authorNiederstraßer, Juliaen
dc.contributor.authorLabouta, Hagar Ibrahimen
dc.contributor.authorMelero, Anaen
dc.contributor.authorGordon, Sarahen
dc.contributor.authorSchneider-Daum, Nicoleen
dc.contributor.authorBals, Roberten
dc.contributor.authorLehr, Claus Michaelen
dc.date.accessioned2018-03-07T15:26:15Z-
dc.date.available2018-03-07T15:26:15Z-
dc.date.issued2017-09-
dc.identifier.citationCalcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. 2017, 118:62-67 Eur J Pharm Biopharmen
dc.identifier.issn1873-3441-
dc.identifier.pmid27888144-
dc.identifier.doi10.1016/j.ejpb.2016.11.026-
dc.identifier.urihttp://hdl.handle.net/10033/621313-
dc.description.abstractThe influence of vitamin D3 and its metabolites calcifediol (25(OH)D) and calcitriol on immune regulation and inflammation is well described, and raises the question of potential benefit against bacterial infections. In the current study, 25(OH)D was encapsulated in liposomes to enable aerosolisation, and tested for the ability to prevent pulmonary infection by Pseudomonas aeruginosa. Prepared 25(OH)D-loaded liposomes were nanosized and monodisperse, with a negative surface charge and a 25(OH)D entrapment efficiency of approximately 23%. Jet nebulisation of liposomes was seen to yield an aerosol suitable for tracheo-bronchial deposition. Interestingly, 25(OH)D in either liposomes or ethanolic solution had no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells (LA-4); treatment of infected, human bronchial 16-HBE cells with 25(OH)D liposomes however resulted in a significant reduction in bacterial survival. Together with the importance of selecting an application-appropriate in vitro model, the current study illustrates the feasibility and practicality of employing liposomes as a means to achieve 25(OH)D lung deposition. 25(OH)D-loaded liposomes further demonstrated promising effects regarding prevention of Pseudomonas infection in human bronchial epithelial cells.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCalcifediol-loaded liposomes for local treatment of pulmonary bacterial infections.en
dc.typeArticleen
dc.contributor.departmentHIPS, Helmholtz-Institut für pharmazeutische Forschung Saarland, Universitätscampus 8.1, 66123 Saarbrücken, Germany.en
dc.identifier.journalEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.Ven
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