2.50
Hdl Handle:
http://hdl.handle.net/10033/621317
Title:
A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus.
Authors:
Resch, Ulrike; Tsatsaronis, James Anthony; Le Rhun, Anaïs; Stübiger, Gerald; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Kasvandik, Sergo; Holzmeister, Susanne; Tinnefeld, Philip; Wai, Sun Nyunt; Charpentier, Emmanuelle
Abstract:
Export of macromolecules via extracellular membrane-derived vesicles (MVs) plays an important role in the biology of Gram-negative bacteria. Gram-positive bacteria have also recently been reported to produce MVs; however, the composition and mechanisms governing vesiculogenesis in Gram-positive bacteria remain undefined. Here, we describe MV production in the Gram-positive human pathogen group A streptococcus (GAS), the etiological agent of necrotizing fasciitis and streptococcal toxic shock syndrome. M1 serotype GAS isolates in culture exhibit MV structures both on the cell wall surface and in the near vicinity of bacterial cells. A comprehensive analysis of MV proteins identified both virulence-associated protein substrates of the general secretory pathway in addition to "anchorless surface proteins." Characteristic differences in the contents, distributions, and fatty acid compositions of specific lipids between MVs and GAS cell membrane were also observed. Furthermore, deep RNA sequencing of vesicular RNAs revealed that GAS MVs contained differentially abundant RNA species relative to bacterial cellular RNA. MV production by GAS strains varied in a manner dependent on an intact two-component system, CovRS, with MV production negatively regulated by the system. Modulation of MV production through CovRS was found to be independent of both GAS cysteine protease SpeB and capsule biosynthesis. Our data provide an explanation for GAS secretion of macromolecules, including RNAs, lipids, and proteins, and illustrate a regulatory mechanism coordinating this secretory response.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus. 2016, 7 (6) MBio
Journal:
mBio
Issue Date:
2016
URI:
http://hdl.handle.net/10033/621317
DOI:
10.1128/mBio.00207-16
PubMed ID:
27803183
Type:
Article
Language:
en
ISSN:
2150-7511
Appears in Collections:
publications of the department Regulation of infection

Full metadata record

DC FieldValue Language
dc.contributor.authorResch, Ulrikeen
dc.contributor.authorTsatsaronis, James Anthonyen
dc.contributor.authorLe Rhun, Anaïsen
dc.contributor.authorStübiger, Geralden
dc.contributor.authorRohde, Manfreden
dc.contributor.authorKasvandik, Sergoen
dc.contributor.authorHolzmeister, Susanneen
dc.contributor.authorTinnefeld, Philipen
dc.contributor.authorWai, Sun Nyunten
dc.contributor.authorCharpentier, Emmanuelleen
dc.date.accessioned2018-03-09T10:47:03Z-
dc.date.available2018-03-09T10:47:03Z-
dc.date.issued2016-
dc.identifier.citationA Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus. 2016, 7 (6) MBioen
dc.identifier.issn2150-7511-
dc.identifier.pmid27803183-
dc.identifier.doi10.1128/mBio.00207-16-
dc.identifier.urihttp://hdl.handle.net/10033/621317-
dc.description.abstractExport of macromolecules via extracellular membrane-derived vesicles (MVs) plays an important role in the biology of Gram-negative bacteria. Gram-positive bacteria have also recently been reported to produce MVs; however, the composition and mechanisms governing vesiculogenesis in Gram-positive bacteria remain undefined. Here, we describe MV production in the Gram-positive human pathogen group A streptococcus (GAS), the etiological agent of necrotizing fasciitis and streptococcal toxic shock syndrome. M1 serotype GAS isolates in culture exhibit MV structures both on the cell wall surface and in the near vicinity of bacterial cells. A comprehensive analysis of MV proteins identified both virulence-associated protein substrates of the general secretory pathway in addition to "anchorless surface proteins." Characteristic differences in the contents, distributions, and fatty acid compositions of specific lipids between MVs and GAS cell membrane were also observed. Furthermore, deep RNA sequencing of vesicular RNAs revealed that GAS MVs contained differentially abundant RNA species relative to bacterial cellular RNA. MV production by GAS strains varied in a manner dependent on an intact two-component system, CovRS, with MV production negatively regulated by the system. Modulation of MV production through CovRS was found to be independent of both GAS cysteine protease SpeB and capsule biosynthesis. Our data provide an explanation for GAS secretion of macromolecules, including RNAs, lipids, and proteins, and illustrate a regulatory mechanism coordinating this secretory response.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshBacterial Proteinsen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshHigh-Throughput Nucleotide Sequencingen
dc.subject.meshIntracellular Signaling Peptides and Proteinsen
dc.subject.meshLipidsen
dc.subject.meshMembrane Proteinsen
dc.subject.meshRNAen
dc.subject.meshRepressor Proteinsen
dc.subject.meshSecretory Vesiclesen
dc.subject.meshStreptococcus pyogenesen
dc.subject.meshVirulence Factorsen
dc.titleA Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalmBioen
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