In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621325
Title:
In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.
Authors:
Villacorta, Luis; Minarrieta, Lucia; Salvatore, Sonia R; Khoo, Nicholas K; Rom, Oren; Gao, Zhen; Berman, Rebecca C; Jobbagy, Soma; Li, Lihua; Woodcock, Steven R; Chen, Y Eugene; Freeman, Bruce A; Ferreira, Ana M; Schopfer, Francisco J; Vitturi, Dario A
Abstract:
Conjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitro-conjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes.
Affiliation:
TWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
Citation:
In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation. 2018, 15:522-531 Redox Biol
Journal:
Redox biology
Issue Date:
May-2018
URI:
http://hdl.handle.net/10033/621325
DOI:
10.1016/j.redox.2018.01.005
PubMed ID:
29413964
Type:
Article
Language:
en
ISSN:
2213-2317
Appears in Collections:
publications of the TwinCore unit Infection immunology

Full metadata record

DC FieldValue Language
dc.contributor.authorVillacorta, Luisen
dc.contributor.authorMinarrieta, Luciaen
dc.contributor.authorSalvatore, Sonia Ren
dc.contributor.authorKhoo, Nicholas Ken
dc.contributor.authorRom, Orenen
dc.contributor.authorGao, Zhenen
dc.contributor.authorBerman, Rebecca Cen
dc.contributor.authorJobbagy, Somaen
dc.contributor.authorLi, Lihuaen
dc.contributor.authorWoodcock, Steven Ren
dc.contributor.authorChen, Y Eugeneen
dc.contributor.authorFreeman, Bruce Aen
dc.contributor.authorFerreira, Ana Men
dc.contributor.authorSchopfer, Francisco Jen
dc.contributor.authorVitturi, Dario Aen
dc.date.accessioned2018-03-19T15:56:35Z-
dc.date.available2018-03-19T15:56:35Z-
dc.date.issued2018-05-
dc.identifier.citationIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation. 2018, 15:522-531 Redox Biolen
dc.identifier.issn2213-2317-
dc.identifier.pmid29413964-
dc.identifier.doi10.1016/j.redox.2018.01.005-
dc.identifier.urihttp://hdl.handle.net/10033/621325-
dc.description.abstractConjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitro-conjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.en
dc.typeArticleen
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalRedox biologyen

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