Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621347
Title:
Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products.
Authors:
Sváb, Domonkos; Falgenhauer, Linda; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Szabó, Judit; Chakraborty, Trinad; Tóth, István
Abstract:
During recent years, interest in the use of bacteriophages as biocontrol agents against foodborne pathogens has increased, particularly for members of the family Enterobacteriaceae, with pathogenic Escherichia coli, Shigella, and Salmonella strains among them. Here, we report the isolation and characterisation of 12 novel T5-like bacteriophages from confiscated food samples. All bacterophages effectively lysed E. coli K-12 strains and were able to infect pathogenic E. coli strains representing enterohaemorrhagic (EHEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes, Shigella dysenteriae, S. sonnei strains, as well as multidrug-resistant (MDR) E. coli and multiple strains representing different Salmonella enterica serovars. All the bacteriophages exhibited Siphoviridae morphology. Whole genome sequencing of the novel T5-like bacteriophages showed that they represent two distinct groups, with the genome-based grouping correlating to the different host spectra. As these bacteriophages are of food origin, their stability and lack of any virulence genes, as well as their broad and mutually complementary host spectrum makes these new T5-like bacteriophages valuable candidates for use as biocontrol agents against foodborne pathogenic enterobacteria.
Affiliation:
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Citation:
Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products. 2018, 9:202 Front Microbiol
Journal:
Frontiers in microbiology
Issue Date:
2018
URI:
http://hdl.handle.net/10033/621347
DOI:
10.3389/fmicb.2018.00202
PubMed ID:
29487585
Type:
Article
Language:
en
ISSN:
1664-302X
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorSváb, Domonkosen
dc.contributor.authorFalgenhauer, Lindaen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorSzabó, Juditen
dc.contributor.authorChakraborty, Trinaden
dc.contributor.authorTóth, Istvánen
dc.date.accessioned2018-04-12T13:16:29Z-
dc.date.available2018-04-12T13:16:29Z-
dc.date.issued2018-
dc.identifier.citationIdentification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products. 2018, 9:202 Front Microbiolen
dc.identifier.issn1664-302X-
dc.identifier.pmid29487585-
dc.identifier.doi10.3389/fmicb.2018.00202-
dc.identifier.urihttp://hdl.handle.net/10033/621347-
dc.description.abstractDuring recent years, interest in the use of bacteriophages as biocontrol agents against foodborne pathogens has increased, particularly for members of the family Enterobacteriaceae, with pathogenic Escherichia coli, Shigella, and Salmonella strains among them. Here, we report the isolation and characterisation of 12 novel T5-like bacteriophages from confiscated food samples. All bacterophages effectively lysed E. coli K-12 strains and were able to infect pathogenic E. coli strains representing enterohaemorrhagic (EHEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes, Shigella dysenteriae, S. sonnei strains, as well as multidrug-resistant (MDR) E. coli and multiple strains representing different Salmonella enterica serovars. All the bacteriophages exhibited Siphoviridae morphology. Whole genome sequencing of the novel T5-like bacteriophages showed that they represent two distinct groups, with the genome-based grouping correlating to the different host spectra. As these bacteriophages are of food origin, their stability and lack of any virulence genes, as well as their broad and mutually complementary host spectrum makes these new T5-like bacteriophages valuable candidates for use as biocontrol agents against foodborne pathogenic enterobacteria.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleIdentification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalFrontiers in microbiologyen

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