2.50
Hdl Handle:
http://hdl.handle.net/10033/84733
Title:
Growth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor.
Authors:
Mehboob, Farrakh; Junca, Howard ( 0000-0003-4546-6229 ) ; Schraa, Gosse; Stams, Alfons J M
Abstract:
Microbial (per)chlorate reduction is a unique process in which molecular oxygen is formed during the dismutation of chlorite. The oxygen thus formed may be used to degrade hydrocarbons by means of oxygenases under seemingly anoxic conditions. Up to now, no bacterium has been described that grows on aliphatic hydrocarbons with chlorate. Here, we report that Pseudomonas chloritidismutans AW-1(T) grows on n-alkanes (ranging from C7 until C12) with chlorate as electron acceptor. Strain AW-1(T) also grows on the intermediates of the presumed n-alkane degradation pathway. The specific growth rates on n-decane and chlorate and n-decane and oxygen were 0.5 +/- 0.1 and 0.4 +/- 0.02 day(-1), respectively. The key enzymes chlorate reductase and chlorite dismutase were assayed and found to be present. The oxygen-dependent alkane oxidation was demonstrated in whole-cell suspensions. The strain degrades n-alkanes with oxygen and chlorate but not with nitrate, thus suggesting that the strain employs oxygenase-dependent pathways for the breakdown of n-alkanes.
Affiliation:
Wageningen University, The Netherlands.
Citation:
Growth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor. 2009, 83 (4):739-47 Appl. Microbiol. Biotechnol.
Journal:
Applied microbiology and biotechnology
Issue Date:
Jun-2009
URI:
http://hdl.handle.net/10033/84733
DOI:
10.1007/s00253-009-1985-9
PubMed ID:
19352644
Type:
Article
Language:
en
ISSN:
1432-0614
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorMehboob, Farrakhen
dc.contributor.authorJunca, Howarden
dc.contributor.authorSchraa, Gosseen
dc.contributor.authorStams, Alfons J Men
dc.date.accessioned2009-10-23T07:38:19Zen
dc.date.available2009-10-23T07:38:19Zen
dc.date.issued2009-06en
dc.identifier.citationGrowth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor. 2009, 83 (4):739-47 Appl. Microbiol. Biotechnol.en
dc.identifier.issn1432-0614en
dc.identifier.pmid19352644en
dc.identifier.doi10.1007/s00253-009-1985-9en
dc.identifier.urihttp://hdl.handle.net/10033/84733en
dc.description.abstractMicrobial (per)chlorate reduction is a unique process in which molecular oxygen is formed during the dismutation of chlorite. The oxygen thus formed may be used to degrade hydrocarbons by means of oxygenases under seemingly anoxic conditions. Up to now, no bacterium has been described that grows on aliphatic hydrocarbons with chlorate. Here, we report that Pseudomonas chloritidismutans AW-1(T) grows on n-alkanes (ranging from C7 until C12) with chlorate as electron acceptor. Strain AW-1(T) also grows on the intermediates of the presumed n-alkane degradation pathway. The specific growth rates on n-decane and chlorate and n-decane and oxygen were 0.5 +/- 0.1 and 0.4 +/- 0.02 day(-1), respectively. The key enzymes chlorate reductase and chlorite dismutase were assayed and found to be present. The oxygen-dependent alkane oxidation was demonstrated in whole-cell suspensions. The strain degrades n-alkanes with oxygen and chlorate but not with nitrate, thus suggesting that the strain employs oxygenase-dependent pathways for the breakdown of n-alkanes.en
dc.language.isoenen
dc.subject.meshAlkanesen
dc.subject.meshChloratesen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshNitratesen
dc.subject.meshOxidoreductasesen
dc.subject.meshOxygenen
dc.subject.meshPseudomonasen
dc.titleGrowth of Pseudomonas chloritidismutans AW-1(T) on n-alkanes with chlorate as electron acceptor.en
dc.typeArticleen
dc.contributor.departmentWageningen University, The Netherlands.en
dc.identifier.journalApplied microbiology and biotechnologyen

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