Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.

2.50
Hdl Handle:
http://hdl.handle.net/10033/90241
Title:
Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.
Authors:
Monciardini, Paolo; Cavaletti, Linda; Ranghetti, Anna; Schumann, Peter; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Bamonte, Ruggiero; Sosio, Margherita; Mezzelani, Alessandra; Donadio, Stefano
Abstract:
Two novel Gram-positive-staining, acidophilic strains were isolated from soil samples. Both show typical features of filamentous actinomycetes. On the basis of 16S rRNA gene sequence analysis, the strains are members of the family Micromonosporaceae. The two strains contain hydroxydiaminopimelic acid, glycine, alanine and glutamic acid in the peptidoglycan. Fatty acid profiles clearly differentiate the two strains: cyclohexyl C(17 : 0), i-C(16 : 0) and ai-C(17 : 0) are predominant in Delta1(T), while the major components for Delta3(T) are ai-C(17 : 0) and i-C(16 : 0). The two strains also differ in their major menaquinones, MK-9(H(8), H(4), H(6)) for Delta1(T) and MK-9(H(8), H(6)) for Delta3(T), and in phospholipid patterns; Delta1(T) displays phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, methyl phosphatidylethanolamine and an unknown aminophospholipid, while Delta3(T) also contains minor amounts of several unknown phospholipids in addition to these phospholipids. The whole-cell sugars of both strains are galactose, arabinose and xylose. The G+C content of the DNA is 72.7 mol% for Delta1(T) and 71.9 mol% for Delta3(T). On the basis of chemotaxonomic, physiological and phylogenetic data, we propose Rugosimonospora gen. nov. to accommodate the two strains, with the description of Rugosimonospora acidiphila gen. nov., sp. nov. (the type species; type strain Delta1(T) =DSM 45227(T) =NBRC 104874(T)) and Rugosimonospora africana sp. nov. (type strain Delta3(T) =DSM 45228(T) =NBRC 104875(T)).
Affiliation:
Vicuron Pharmaceuticals, 21040 Gerenzano, Italy. paolo.monciardini@ktedogen.com
Citation:
Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov. 2009, 59 (Pt 11):2752-8 Int. J. Syst. Evol. Microbiol.
Journal:
International journal of systematic and evolutionary microbiology
Issue Date:
Nov-2009
URI:
http://hdl.handle.net/10033/90241
DOI:
10.1099/ijs.0.010231-0
PubMed ID:
19625428
Type:
Article
Language:
en
ISSN:
1466-5026
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorMonciardini, Paoloen
dc.contributor.authorCavaletti, Lindaen
dc.contributor.authorRanghetti, Annaen
dc.contributor.authorSchumann, Peteren
dc.contributor.authorRohde, Manfreden
dc.contributor.authorBamonte, Ruggieroen
dc.contributor.authorSosio, Margheritaen
dc.contributor.authorMezzelani, Alessandraen
dc.contributor.authorDonadio, Stefanoen
dc.date.accessioned2010-01-21T10:38:08Zen
dc.date.available2010-01-21T10:38:08Zen
dc.date.issued2009-11en
dc.identifier.citationNovel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov. 2009, 59 (Pt 11):2752-8 Int. J. Syst. Evol. Microbiol.en
dc.identifier.issn1466-5026en
dc.identifier.pmid19625428en
dc.identifier.doi10.1099/ijs.0.010231-0en
dc.identifier.urihttp://hdl.handle.net/10033/90241en
dc.description.abstractTwo novel Gram-positive-staining, acidophilic strains were isolated from soil samples. Both show typical features of filamentous actinomycetes. On the basis of 16S rRNA gene sequence analysis, the strains are members of the family Micromonosporaceae. The two strains contain hydroxydiaminopimelic acid, glycine, alanine and glutamic acid in the peptidoglycan. Fatty acid profiles clearly differentiate the two strains: cyclohexyl C(17 : 0), i-C(16 : 0) and ai-C(17 : 0) are predominant in Delta1(T), while the major components for Delta3(T) are ai-C(17 : 0) and i-C(16 : 0). The two strains also differ in their major menaquinones, MK-9(H(8), H(4), H(6)) for Delta1(T) and MK-9(H(8), H(6)) for Delta3(T), and in phospholipid patterns; Delta1(T) displays phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, methyl phosphatidylethanolamine and an unknown aminophospholipid, while Delta3(T) also contains minor amounts of several unknown phospholipids in addition to these phospholipids. The whole-cell sugars of both strains are galactose, arabinose and xylose. The G+C content of the DNA is 72.7 mol% for Delta1(T) and 71.9 mol% for Delta3(T). On the basis of chemotaxonomic, physiological and phylogenetic data, we propose Rugosimonospora gen. nov. to accommodate the two strains, with the description of Rugosimonospora acidiphila gen. nov., sp. nov. (the type species; type strain Delta1(T) =DSM 45227(T) =NBRC 104874(T)) and Rugosimonospora africana sp. nov. (type strain Delta3(T) =DSM 45228(T) =NBRC 104875(T)).en
dc.language.isoenen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Ribosomalen
dc.subject.meshFatty Acidsen
dc.subject.meshMicromonosporaceaeen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPhylogenyen
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshSoil Microbiologyen
dc.titleNovel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.en
dc.typeArticleen
dc.contributor.departmentVicuron Pharmaceuticals, 21040 Gerenzano, Italy. paolo.monciardini@ktedogen.comen
dc.identifier.journalInternational journal of systematic and evolutionary microbiologyen

Related articles on PubMed

All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.