Bifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host.

2.50
Hdl Handle:
http://hdl.handle.net/10033/92942
Title:
Bifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host.
Authors:
Candela, Marco; Biagi, Elena; Centanni, Manuela; Turroni, Silvia; Vici, Manuela; Musiani, Francesco; Vitali, Beatrice; Bergmann, Simone; Hammerschmidt, Sven; Brigidi, Patrizia
Abstract:
The interaction with the host plasminogen/plasmin system represents a novel component in the molecular cross-talk between bifidobacteria and human host. Here, we demonstrated that the plasminogen-binding bifidobacterial species B. longum, B. bifidum, B. breve and B. lactis share the key glycolytic enzyme enolase as a surface receptor for human plasminogen. Enolase was visualized on the cell surface of the model strain B. lactis BI07. The His-tagged recombinant protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. By site-directed mutagenesis we demonstrated that the interaction between the B. lactis BI07 enolase and human plasminogen involves an internal plasminogen-binding site homologous to that of pneumococcal enolase. According to our data, the positively charged residues Lys-251 and Lys-255, as well as the negatively charged Glu-252, of the B. lactis BI07 enolase are crucial for plasminogen binding. Acting as a human plasminogen receptor, the bifidobacterial surface enolase is suggested to play an important role in the interaction process with the host.
Affiliation:
Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Italy.
Citation:
Bifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host. 2009, 155 (Pt 10):3294-303 Microbiology (Reading, Engl.)
Journal:
Microbiology (Reading, England)
Issue Date:
Oct-2009
URI:
http://hdl.handle.net/10033/92942
DOI:
10.1099/mic.0.028795-0
PubMed ID:
19574304
Type:
Article
Language:
en
ISSN:
1350-0872
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorCandela, Marcoen
dc.contributor.authorBiagi, Elenaen
dc.contributor.authorCentanni, Manuelaen
dc.contributor.authorTurroni, Silviaen
dc.contributor.authorVici, Manuelaen
dc.contributor.authorMusiani, Francescoen
dc.contributor.authorVitali, Beatriceen
dc.contributor.authorBergmann, Simoneen
dc.contributor.authorHammerschmidt, Svenen
dc.contributor.authorBrigidi, Patriziaen
dc.date.accessioned2010-02-24T14:25:06Z-
dc.date.available2010-02-24T14:25:06Z-
dc.date.issued2009-10-
dc.identifier.citationBifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host. 2009, 155 (Pt 10):3294-303 Microbiology (Reading, Engl.)en
dc.identifier.issn1350-0872-
dc.identifier.pmid19574304-
dc.identifier.doi10.1099/mic.0.028795-0-
dc.identifier.urihttp://hdl.handle.net/10033/92942-
dc.description.abstractThe interaction with the host plasminogen/plasmin system represents a novel component in the molecular cross-talk between bifidobacteria and human host. Here, we demonstrated that the plasminogen-binding bifidobacterial species B. longum, B. bifidum, B. breve and B. lactis share the key glycolytic enzyme enolase as a surface receptor for human plasminogen. Enolase was visualized on the cell surface of the model strain B. lactis BI07. The His-tagged recombinant protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. By site-directed mutagenesis we demonstrated that the interaction between the B. lactis BI07 enolase and human plasminogen involves an internal plasminogen-binding site homologous to that of pneumococcal enolase. According to our data, the positively charged residues Lys-251 and Lys-255, as well as the negatively charged Glu-252, of the B. lactis BI07 enolase are crucial for plasminogen binding. Acting as a human plasminogen receptor, the bifidobacterial surface enolase is suggested to play an important role in the interaction process with the host.en
dc.language.isoenen
dc.subject.meshBifidobacteriumen
dc.subject.meshBinding Sitesen
dc.subject.meshDNA, Bacterialen
dc.subject.meshHost-Pathogen Interactionsen
dc.subject.meshHumansen
dc.subject.meshKineticsen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMutagenesis, Site-Directeden
dc.subject.meshPhosphopyruvate Hydrataseen
dc.subject.meshPlasminogenen
dc.subject.meshProtein Bindingen
dc.subject.meshProtein Interaction Mappingen
dc.subject.meshSequence Analysis, DNAen
dc.titleBifidobacterial enolase, a cell surface receptor for human plasminogen involved in the interaction with the host.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Italy.en
dc.identifier.journalMicrobiology (Reading, England)en

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