SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.

2.50
Hdl Handle:
http://hdl.handle.net/10033/94093
Title:
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Authors:
Klebensberger, Janosch; Birkenmaier, Antoinette; Geffers, Robert; Kjelleberg, Staffan; Philipp, Bodo
Abstract:
Cell aggregation is a stress response and serves as a survival strategy for Pseudomonas aeruginosa strain PAO1 during growth with the toxic detergent Na-dodecylsulfate (SDS). This process involves the psl operon and is linked to c-di-GMP signalling. The induction of cell aggregation in response to SDS was studied. Transposon and site-directed mutagenesis revealed that the cupA-operon and the co-transcribed genes siaA (PA0172) and siaD (PA0169) were essential for SDS-induced aggregation. While siaA encodes a putative membrane protein with a HAMP and a PP2C-like phosphatase domain, siaD encodes a putative diguanylate cyclase involved in the biosynthesis of c-di-GMP. Complementation studies uncovered that the loss of SDS-induced aggregation in the formerly isolated spontaneous mutant strain N was caused by a non-functional siaA allele. DNA-microarray analysis of SDS-grown cells revealed consistent activation of eight genes, including cupA1, with known or presumptive important functions in cell aggregation in the parent strain compared with non-aggregating siaA and siaD mutants. A siaAD-dependent increase of cupA1 mRNA levels in SDS-grown cells was also shown by Northern blots. These results clearly demonstrate that SiaAD are essential for inducing cell aggregation as a specific response to SDS and suggest that they are responsible for perceiving and transducing SDS-related stress.
Affiliation:
Universität Konstanz, Fachbereich Biologie, Mikrobielle Okologie, Fach M654, 78457 Konstanz, Germany.
Citation:
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa. 2009, 11 (12):3073-86 Environ. Microbiol.
Journal:
Environmental microbiology
Issue Date:
Dec-2009
URI:
http://hdl.handle.net/10033/94093
DOI:
10.1111/j.1462-2920.2009.02012.x
PubMed ID:
19638175
Type:
Article
Language:
en
ISSN:
1462-2920
Appears in Collections:
Publications of Dept. Cell Biology (ZB)

Full metadata record

DC FieldValue Language
dc.contributor.authorKlebensberger, Janoschen
dc.contributor.authorBirkenmaier, Antoinetteen
dc.contributor.authorGeffers, Roberten
dc.contributor.authorKjelleberg, Staffanen
dc.contributor.authorPhilipp, Bodoen
dc.date.accessioned2010-03-11T09:20:53Z-
dc.date.available2010-03-11T09:20:53Z-
dc.date.issued2009-12-
dc.identifier.citationSiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa. 2009, 11 (12):3073-86 Environ. Microbiol.en
dc.identifier.issn1462-2920-
dc.identifier.pmid19638175-
dc.identifier.doi10.1111/j.1462-2920.2009.02012.x-
dc.identifier.urihttp://hdl.handle.net/10033/94093-
dc.description.abstractCell aggregation is a stress response and serves as a survival strategy for Pseudomonas aeruginosa strain PAO1 during growth with the toxic detergent Na-dodecylsulfate (SDS). This process involves the psl operon and is linked to c-di-GMP signalling. The induction of cell aggregation in response to SDS was studied. Transposon and site-directed mutagenesis revealed that the cupA-operon and the co-transcribed genes siaA (PA0172) and siaD (PA0169) were essential for SDS-induced aggregation. While siaA encodes a putative membrane protein with a HAMP and a PP2C-like phosphatase domain, siaD encodes a putative diguanylate cyclase involved in the biosynthesis of c-di-GMP. Complementation studies uncovered that the loss of SDS-induced aggregation in the formerly isolated spontaneous mutant strain N was caused by a non-functional siaA allele. DNA-microarray analysis of SDS-grown cells revealed consistent activation of eight genes, including cupA1, with known or presumptive important functions in cell aggregation in the parent strain compared with non-aggregating siaA and siaD mutants. A siaAD-dependent increase of cupA1 mRNA levels in SDS-grown cells was also shown by Northern blots. These results clearly demonstrate that SiaAD are essential for inducing cell aggregation as a specific response to SDS and suggest that they are responsible for perceiving and transducing SDS-related stress.en
dc.language.isoenen
dc.subject.meshDetergentsen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenes, Bacterialen
dc.subject.meshMutationen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshSodium Dodecyl Sulfateen
dc.subject.meshStress, Physiologicalen
dc.titleSiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentUniversität Konstanz, Fachbereich Biologie, Mikrobielle Okologie, Fach M654, 78457 Konstanz, Germany.en
dc.identifier.journalEnvironmental microbiologyen
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