2.50
Hdl Handle:
http://hdl.handle.net/10033/97979
Title:
Molecular dissection of Salmonella-induced membrane ruffling versus invasion.
Authors:
Hänisch, Jan; Ehinger, Julia; Ladwein, Markus; Rohde, Manfred; Derivery, Emmanuel; Bosse, Tanja; Steffen, Anika; Bumann, Dirk; Misselwitz, Benjamin; Hardt, Wolf-Dietrich; Gautreau, Alexis; Stradal, Theresia E B; Rottner, Klemens
Abstract:
Type III secretion system-mediated injection of a cocktail of bacterial proteins drives actin rearrangements, frequently adopting the shape of prominent protuberances of ruffling membrane, and culminating in host cell invasion of Gram-negative pathogens like Salmonella typhimurium. Different Salmonella effectors are able to bind actin and activate Rho-family GTPases, which have previously been implicated in mediating actin-dependent Salmonella entry by interacting with N-WASP or WAVE-complex, well-established activators of the actin nucleation machine Arp2/3-complex. Using genetic deletion and RNA interference studies, we show here that neither individual nor collective removal of these Arp2/3- complex activators affected host cell invasion as efficiently as Arp2/3-complex knock-down, although the latter was also not essential. However, interference with WAVE-complex function abrogated Salmonella-induced membrane ruffling without significantly affecting entry efficiency, actin or Arp2/3-complex accumulation. In addition, scanning electron microscopy images captured entry events in the absence of prominent membrane ruffles. Finally, localization and RNA interference studies indicated a relevant function in Salmonella entry for the novel Arp2/3-complex regulator WASH. These data establish for the first time that Salmonella invasion is separable from bacteria-induced membrane ruffling, and uncover an additional Arp2/3-complex activator as well as an Arp2/3-complex-independent actin assembly activity that contribute to Salmonella invasion.
Affiliation:
Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Molecular dissection of Salmonella-induced membrane ruffling versus invasion. 2010, 12 (1):84-98 Cell. Microbiol.
Journal:
Cellular microbiology
Issue Date:
Jan-2010
URI:
http://hdl.handle.net/10033/97979
DOI:
10.1111/j.1462-5822.2009.01380.x
PubMed ID:
19732055
Type:
Article
Language:
en
ISSN:
1462-5822
Appears in Collections:
Publications of RG Cytoskeleton Dynamics (CYD)

Full metadata record

DC FieldValue Language
dc.contributor.authorHänisch, Janen
dc.contributor.authorEhinger, Juliaen
dc.contributor.authorLadwein, Markusen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorDerivery, Emmanuelen
dc.contributor.authorBosse, Tanjaen
dc.contributor.authorSteffen, Anikaen
dc.contributor.authorBumann, Dirken
dc.contributor.authorMisselwitz, Benjaminen
dc.contributor.authorHardt, Wolf-Dietrichen
dc.contributor.authorGautreau, Alexisen
dc.contributor.authorStradal, Theresia E Ben
dc.contributor.authorRottner, Klemensen
dc.date.accessioned2010-05-05T13:49:53Z-
dc.date.available2010-05-05T13:49:53Z-
dc.date.issued2010-01-
dc.identifier.citationMolecular dissection of Salmonella-induced membrane ruffling versus invasion. 2010, 12 (1):84-98 Cell. Microbiol.en
dc.identifier.issn1462-5822-
dc.identifier.pmid19732055-
dc.identifier.doi10.1111/j.1462-5822.2009.01380.x-
dc.identifier.urihttp://hdl.handle.net/10033/97979-
dc.description.abstractType III secretion system-mediated injection of a cocktail of bacterial proteins drives actin rearrangements, frequently adopting the shape of prominent protuberances of ruffling membrane, and culminating in host cell invasion of Gram-negative pathogens like Salmonella typhimurium. Different Salmonella effectors are able to bind actin and activate Rho-family GTPases, which have previously been implicated in mediating actin-dependent Salmonella entry by interacting with N-WASP or WAVE-complex, well-established activators of the actin nucleation machine Arp2/3-complex. Using genetic deletion and RNA interference studies, we show here that neither individual nor collective removal of these Arp2/3- complex activators affected host cell invasion as efficiently as Arp2/3-complex knock-down, although the latter was also not essential. However, interference with WAVE-complex function abrogated Salmonella-induced membrane ruffling without significantly affecting entry efficiency, actin or Arp2/3-complex accumulation. In addition, scanning electron microscopy images captured entry events in the absence of prominent membrane ruffles. Finally, localization and RNA interference studies indicated a relevant function in Salmonella entry for the novel Arp2/3-complex regulator WASH. These data establish for the first time that Salmonella invasion is separable from bacteria-induced membrane ruffling, and uncover an additional Arp2/3-complex activator as well as an Arp2/3-complex-independent actin assembly activity that contribute to Salmonella invasion.en
dc.language.isoenen
dc.subject.meshActin-Related Protein 2-3 Complexen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBlotting, Westernen
dc.subject.meshCell Lineen
dc.subject.meshCell Membraneen
dc.subject.meshFibroblastsen
dc.subject.meshHumansen
dc.subject.meshMicroscopy, Electron, Scanningen
dc.subject.meshMicroscopy, Fluorescenceen
dc.subject.meshProteinsen
dc.subject.meshRNA Interferenceen
dc.subject.meshSalmonella typhimuriumen
dc.subject.meshWiskott-Aldrich Syndrome Protein, Neuronalen
dc.titleMolecular dissection of Salmonella-induced membrane ruffling versus invasion.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Braunschweig, Germany.en
dc.identifier.journalCellular microbiologyen

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